Dbf4 Antibody (6G9): sc-293398
- Dbf4 Antibody (6G9) is a mouse monoclonal IgG1 κ Dbf4 antibody provided at 100 µg/ml
- raised against amino acids 2-98 of Dbf4 of human origin
- recommended for detection of Dbf4 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Dbf4 Antibody (6G9). This work is in progress.
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Dbf4 Antibody (6G9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dbf4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Dbf4 antibody (6G9) is available as the non-conjugated format. Dbf4 (6G9) antibody recognizes a protein that plays a crucial role in regulating DNA replication, specifically in forming and activating the Dbf4/Cdc7 kinase complex, which is essential for initiating DNA replication during the S phase of the cell cycle. This complex phosphorylates key proteins necessary for DNA replication initiation, including several minichromosome maintenance proteins and the p180 subunit of DNA polymerase alpha-primase. Dbf4 expression remains tightly regulated throughout the cell cycle, staying absent during G1 phase but accumulating during S and G2 phases, ensuring DNA replication occurs at appropriate times. Timely degradation of Dbf4 by the anaphase-promoting complex during chromosome segregation prevents premature reactivation of prereplicative complexes, maintaining genomic stability. Dbf4 monoclonal antibody (6G9) effectively detects this important regulatory protein, making this reagent valuable for studying cell cycle dynamics and DNA replication mechanisms.
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Dbf4 Antibody (6G9) References:
- A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication. | Pasero, P., et al. 1999. Genes Dev. 13: 2159-76. PMID: 10465792
- Cdc7p-Dbf4p kinase binds to chromatin during S phase and is regulated by both the APC and the RAD53 checkpoint pathway. | Weinreich, M. and Stillman, B. 1999. EMBO J. 18: 5334-46. PMID: 10508166
- Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. | Lepke, M., et al. 1999. Mol Gen Genet. 262: 220-9. PMID: 10517317
- Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication. | Jiang, W., et al. 1999. EMBO J. 18: 5703-13. PMID: 10523313
- CDC7 kinase complex as a molecular switch for DNA replication. | Masai, H., et al. 1999. Front Biosci. 4: D834-40. PMID: 10577390
- Dbf4p, an essential S phase-promoting factor, is targeted for degradation by the anaphase-promoting complex. | Ferreira, MF., et al. 2000. Mol Cell Biol. 20: 242-8. PMID: 10594027
- Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. | Tsuji, T., et al. 2006. Mol Biol Cell. 17: 4459-72. PMID: 16899510
- Cdc7-Dbf4 and the human S checkpoint response to UVC. | Heffernan, TP., et al. 2007. J Biol Chem. 282: 9458-9468. PMID: 17276990
- The Cdc7 protein kinase is required for origin firing during S phase. | Bousset, K. and Diffley, JF. 1998. Genes Dev. 12: 480-90. PMID: 9472017
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dbf4 Antibody (6G9) | sc-293398 | 100 µg/ml | $316.00 |