DAX-1 Double Nickase Plasmid (h): sc-402180-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAX-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DAX-1 Double Nickase Plasmid (h) and DAX-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR0B1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAX-1 Antibody (3G8): sc-293452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAX-1 Double Nickase Plasmid (h) | sc-402180-NIC | 20 µg | $410.00 | |||
DAX-1 Double Nickase Plasmid (h2) | sc-402180-NIC-2 | 20 µg | $410.00 |
NR0B1 encodes DAX-1 (NR0B1), an atypical orphan nuclear receptor that functions primarily as a transcriptional coregulator rather than a classical DNA-binding receptor. DAX-1 modulates steroidogenic gene networks by interacting with nuclear receptors and coregulators to regulate pathways controlling adrenal and gonadal development, steroid hormone biosynthesis, and hypothalamic–pituitary–gonadal axis signaling. In human cells, NR0B1 influences lineage specification and endocrine homeostasis through transcriptional repression/activation programs affecting SF-1/NR5A1- and LRH-1/NR5A2-associated regulatory circuits. Dysregulation of NR0B1 is linked to disorders of sex development and adrenal insufficiency phenotypes, supporting its utility as a target in mechanistic studies of endocrine development and nuclear receptor signaling.
DAX-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR0B1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR0B1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR0B1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR0B1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.