DAX-1 CRISPR/Cas9 KO Plasmid (m): sc-419053
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAX-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DAX-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAX-1 CRISPR/Cas9 KO Plasmid (m) | sc-419053 | 20 µg | $397.00 |
Overview
Nr0b1 encodes DAX-1 (NR0B1), an atypical orphan nuclear receptor that functions primarily as a transcriptional coregulator in steroidogenic and reproductive tissues. DAX-1 modulates nuclear receptor signaling networks by repressing or fine-tuning transcriptional programs controlled by factors such as SF-1/NR5A1, thereby influencing steroid hormone biosynthesis, adrenal development, and hypothalamic–pituitary–gonadal axis regulation. In mouse models, altered DAX-1 activity impacts gonadal differentiation, gametogenesis, and endocrine homeostasis, making Nr0b1 a relevant node for studying developmental gene regulation and steroidogenic pathway control. Dysregulation of NR0B1 orthologs has been linked to congenital adrenal and reproductive phenotypes, supporting its use in mechanistic studies of endocrine development and sex determination pathways.
DAX-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nr0b1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nr0b1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nr0b1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DAX-1 protein expression.
This CRISPR knockout system enables efficient generation of Nr0b1-deficient cell models for investigation of DAX-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nr0b1 exon(s) critical for DAX-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nr0b1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DAX-1 CRISPR/Cas9 KO Plasmid (m) and DAX-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nr0b1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DAX-1 HDR Plasmid (m) and DAX-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nr0b1 homology arms to support homology-directed repair at defined Nr0b1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.