Date published: 2025-12-13

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DAT/Dopamine Transporter/SLC6A3 Antibody (hDAT-LOOP): sc-58517

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Datasheets
  • DAT/Dopamine Transporter/SLC6A3 Antibody (hDAT-LOOP) is a rat monoclonal IgG2a provided at 200 µg/ml
  • raised against amino acids 180-218 corresponding to the second extracellular loop of DAT of human origin
  • recommended for detection of DAT of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See DAT/Dopamine Transporter/SLC6A3 (6-8D6): sc-32259 for DAT/Dopamine Transporter/SLC6A3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DAT/Dopamine Transporter/SLC6A3 Antibody (hDAT-LOOP). This work is in progress.

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    DAT Antibody (hDAT-LOOP) is a rat monoclonal IgG2a antibody that detects dopamine transporter (DAT) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-DAT antibody (hDAT-LOOP) plays a crucial role in studying the regulation of dopamine levels in the synaptic cleft, which is essential for maintaining proper neurotransmission and overall brain function. DAT (hDAT-LOOP) monoclonal antibody facilitates research on dopamine reuptake from the synaptic cleft back into presynaptic neurons, helping to terminate dopamine signaling and prevent excessive stimulation of dopamine receptors. This process is particularly important in studying neuropsychiatric disorders, where dysregulation of dopamine signaling is implicated in conditions such as schizophrenia, depression, and addiction. DAT (hDAT-LOOP) antibody provides reliable detection and characterization of DAT, enabling researchers to explore its role in various physiological and pathological processes, including interactions with other proteins that modulate dopamine signaling and its potential as a therapeutic target in dopamine-related disorders.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    DAT/Dopamine Transporter/SLC6A3 Antibody (hDAT-LOOP)

    sc-58517
    200 µg/ml
    $316.00

    I am using DAT (hDAT-LOOP): sc-58517 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

    Asked by: Germaine
    We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-24
    • y_2025, m_11, d_13, h_10CST
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    Rated 5 out of 5 by from Really nice IHC staining!Great antibody for IHC on paraffin sections and WB. Specific and reliable, it's a smart choice for DAT detection.
    Date published: 2017-01-27
    Rated 4 out of 5 by from Produced clear immunoperoxidase cytoplasmicProduced clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human hippocampus tissue. -SCBT QC
    Date published: 2015-06-26
    Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong band detected in SK-N-SH whole cell lysate. -SCBT QC
    Date published: 2015-01-25
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    DAT/Dopamine Transporter/SLC6A3 Antibody (hDAT-LOOP) is rated 4.7 out of 5 by 3.
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