DAPK Antibody (17): sc-136286

DAPK Antibody (17) is a mouse monoclonal IgG1 kappa light chain antibody that detects DAPK protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-DAPK antibody (17) is available as the non-conjugated format. DAPK protein, or death-associated protein kinase, plays a crucial role in the regulation of apoptosis, a vital process for maintaining cellular homeostasis and preventing tumorigenesis. DAPK is characterized by its "death domain," which is essential for mediating apoptosis in response to various stimuli, including interferon-gamma. Introduction of DAPK into highly metastatic carcinoma clones lacking DAPK expression has been shown to suppress metastasis, highlighting DAPK′s significant role in cancer biology. DAPK interacts with other proteins involved in apoptotic signaling pathways, underscoring DAPK′s importance in cellular responses to stress and damage. DAPK′s ability to influence cell fate decisions makes DAPK a critical target for research in cancer therapeutics and understanding of apoptotic mechanisms.

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DAPK Antibody (17) References:

1. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK.  |  Jin, Y., et al. 2002. J Biol Chem. 277: 46980-6. PMID: 12351649
2. DAPK catalytic activity in the hippocampus increases during the recovery phase in an animal model of brain hypoxic-ischemic injury.  |  Schumacher, AM., et al. 2002. Biochim Biophys Acta. 1600: 128-37. PMID: 12445468
3. Frequent promoter methylation of CDH1, DAPK, RARB, and HIC1 genes in carcinoma of cervix uteri: its relationship to clinical outcome.  |  Narayan, G., et al. 2003. Mol Cancer. 2: 24. PMID: 12773202
4. Expression of a novel type I keratin, DAPK-1 in the dorsal aorta and pronephric duct of the zebrafish embryos.  |  Jang, WS., et al. 2003. Gene. 312: 145-50. PMID: 12909350
5. Promoter methylation and down-regulation of DAPK is associated with gastric atrophy.  |  Kim, WS., et al. 2003. Int J Mol Med. 12: 827-30. PMID: 14612952
6. Bidirectional signals transduced by DAPK-ERK interaction promote the apoptotic effect of DAPK.  |  Chen, CH., et al. 2005. EMBO J. 24: 294-304. PMID: 15616583
7. Assignment of DAP1 and DAPK--genes that positively mediate programmed cell death triggered by IFN-gamma--to chromosome regions 5p12.2 and 9q34.1, respectively.  |  Feinstein, E., et al. 1995. Genomics. 29: 305-7. PMID: 8530096
8. DAP kinase links the control of apoptosis to metastasis.  |  Inbal, B., et al. 1997. Nature. 390: 180-4. PMID: 9367156
9. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis.  |  Kawai, T., et al. 1998. Mol Cell Biol. 18: 1642-51. PMID: 9488481
10. Molecular cloning and developmental expression of a rat homologue of death-associated protein kinase in the nervous system.  |  Sakagami, H. and Kondo, H. 1997. Brain Res Mol Brain Res. 52: 249-56. PMID: 9495546

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAPK Antibody (17) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-DAPK Antibody (17) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAPK Antibody (17) Specifications

Alternate Product Name: DAPK

Product Clone Name: 17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 694-947

Epitope Target: DAPK

Epitope Species: human

Product Reactivity/Detection: DAPK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for DAPK Antibody (17): N/A