DAP12 Antibody (B-2): sc-166086
- DAP12 Antibody (B-2) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 76-112 at the C-terminus of DAP12 of human origin
- recommended for detection of DAP12 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for DAP12 Antibody (B-2) for WB applications. This reagent is now offered in a bundle with DAP12 Antibody (B-2) (see ordering information below).
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DAP12 Antibody (B-2) is a mouse monoclonal IgG2b antibody that detects DAP12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP12 is a crucial phosphoprotein that plays a significant role in the activation of natural killer (NK) cells, which are essential components of the immune system responsible for identifying and eliminating infected or cancerous cells. DAP12 is primarily located in the plasma membrane of NK cells, where DAP12 interacts with various receptor families, including killer-cell inhibitory receptors (KIR) and CD94/NKG2C, to modulate immune responses. This membrane localization is vital as DAP12 effectively transmits activating signals upon receptor engagement, thereby enhancing NK cell activity. Furthermore, DAP12′s ability to bind to other proteins, such as ZAP-70 and Syk, upon phosphorylation indicates shared activation pathways with T and B cell antigen receptors, underscoring DAP12′s importance in orchestrating immune responses. Anti-DAP12 antibody (B-2) is an invaluable tool for researchers studying NK cell biology and the intricate signaling mechanisms that govern immune activation.
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DAP12 Antibody (B-2) References:
- TREM2, a DAP12-associated receptor, regulates osteoclast differentiation and function. | Humphrey, MB., et al. 2006. J Bone Miner Res. 21: 237-45. PMID: 16418779
- DAP12 signaling regulates plasmacytoid dendritic cell homeostasis and down-modulates their function during viral infection. | Sjölin, H., et al. 2006. J Immunol. 177: 2908-16. PMID: 16920926
- TREM2- and DAP12-dependent activation of PI3K requires DAP10 and is inhibited by SHIP1. | Peng, Q., et al. 2010. Sci Signal. 3: ra38. PMID: 20484116
- Cytoskeletal dysfunction dominates in DAP12-deficient osteoclasts. | Zou, W., et al. 2010. J Cell Sci. 123: 2955-63. PMID: 20720152
- DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation. | Spahn, JH., et al. 2015. J Immunol. 194: 4039-48. PMID: 25762783
- Variable expression of microglial DAP12 and TREM2 genes in Nasu-Hakola disease. | Sasaki, A., et al. 2015. Neurogenetics. 16: 265-76. PMID: 26001891
- TREM2/DAP12 Signal Elicits Proinflammatory Response in Microglia and Exacerbates Neuropathic Pain. | Kobayashi, M., et al. 2016. J Neurosci. 36: 11138-11150. PMID: 27798193
- TREM2/DAP12 Complex Regulates Inflammatory Responses in Microglia via the JNK Signaling Pathway. | Zhong, L., et al. 2017. Front Aging Neurosci. 9: 204. PMID: 28680398
- CSTA plays a role in osteoclast formation and bone resorption by mediating the DAP12/TREM2 pathway. | Wei, R., et al. 2022. Biochem Biophys Res Commun. 627: 12-20. PMID: 36007331
- Huang-Lian-Jie-Du decoction alleviates depressive-like behaviors in dextran sulfate sodium-induced colitis mice via Trem2/Dap12 pathway. | Zheng, JY., et al. 2023. J Ethnopharmacol. 315: 116658. PMID: 37263316
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAP12 Antibody (B-2) | sc-166086 | 200 µg/ml | $322.00 | |||
DAP12 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-540011 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAP12 (B-2) Neutralizing Peptide | sc-166086 P | 100 µg/0.5 ml | $69.00 |