DAP12 Antibody (A-8): sc-166085
- DAP12 Antibody (A-8) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 76-112 at the C-terminus of DAP12 of human origin
- recommended for detection of DAP12 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for DAP12 Antibody (A-8) for WB applications. These reagents are now offered in bundles with DAP12 Antibody (A-8) (see ordering information below).
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DAP12 Antibody (A-8) is a mouse monoclonal IgG2a antibody that detects DAP12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP12 is a crucial phosphoprotein that plays a significant role in the activation of natural killer (NK) cells, which are essential components of the immune system responsible for identifying and eliminating infected or cancerous cells. DAP12′s ability to interact with various membrane glycoproteins, particularly those belonging to the killer-cell inhibitory receptor (KIR) family, is vital for mediating cellular activation and ensuring a balanced immune response. Additionally, DAP12′s association with the activating NK cell receptor CD94/NKG2C, part of the C-type lectin superfamily, highlights DAP12′s importance in enhancing NK cell activity. The phosphorylation of DAP12 allows binding to signaling proteins such as ZAP-70 and Syk, indicating that DAP12′s activation pathway may share similarities with those of T and B cell antigen receptors, thereby underscoring DAP12′s critical role in immune signaling and response. Anti-DAP12 antibody (A-8) is an invaluable tool for researchers studying NK cell biology and the intricate mechanisms of immune regulation.
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DAP12 Antibody (A-8) References:
- DAP12 and KAP10 (DAP10)-novel transmembrane adapter proteins of the CD3zeta family. | Wilson, MJ., et al. 2000. Immunol Res. 22: 21-42. PMID: 10945225
- DAP12 signaling: from immune cells to bone modeling and brain myelination. | Colonna, M. 2003. J Clin Invest. 111: 313-4. PMID: 12569153
- Role of DAP12 in innate and adaptive immune responses. | Aoki, N., et al. 2003. Curr Pharm Des. 9: 7-10. PMID: 12570670
- The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses. | Nakano-Yokomizo, T., et al. 2011. J Exp Med. 208: 1661-71. PMID: 21727189
- Environmental factors determine DAP12 deficiency to either enhance or suppress immunopathogenic processes. | Montalvo, V., et al. 2013. Immunology. 140: 475-82. PMID: 23906311
- DAP12-based activating chimeric antigen receptor for NK cell tumor immunotherapy. | Töpfer, K., et al. 2015. J Immunol. 194: 3201-12. PMID: 25740942
- Variable expression of microglial DAP12 and TREM2 genes in Nasu-Hakola disease. | Sasaki, A., et al. 2015. Neurogenetics. 16: 265-76. PMID: 26001891
- TREM1/Dap12-based CAR-T cells show potent antitumor activity. | Chen, B., et al. 2019. Immunotherapy. 11: 1043-1055. PMID: 31268375
- DAP12 deletion causes age-related motor function impairment but promotes functional recovery after sciatic nerve crush injury. | Liu, C., et al. 2023. Exp Neurol. 360: 114296. PMID: 36503041
- A novel TREM1/DAP12-based multiple chain CAR-T cell targets PTK7 in ovarian cancer therapy. | Xu, T., et al. 2023. Med Oncol. 40: 226. PMID: 37405498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAP12 Antibody (A-8) | sc-166085 | 200 µg/ml | $322.00 | |||
DAP12 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-540010 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAP12 Antibody (A-8): m-IgG2a BP-HRP Bundle | sc-546836 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAP12 (A-8) Neutralizing Peptide | sc-166085 P | 100 µg/0.5 ml | $69.00 |