DAGLβ Antibody (A-5): sc-514738
- DAGLβ Antibody (A-5) is a mouse monoclonal IgG1 λ DAGLβ antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 554-569 within a C-terminal cytoplasmic domain of DAGLβ of human origin
- DAGL beta Antibody (A-5) is recommended for detection of DAGLβ isoforms 1 and 2 of human origin by WB, IP, IF and ELISA
- Anti-DAGL beta Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for DAGLβ Antibody (A-5) for WB applications. These reagents are now offered in bundles with DAGLβ Antibody (A-5) (see ordering information below).
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DAGLβ Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects the DAGL beta protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAGLβ Antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAGLβ, also known as diacylglycerol lipase beta or KCCR13L, is a 672 amino acid multi-pass membrane protein that plays a pivotal role in lipid metabolism as a member of the AB hydrolase superfamily. DAGLβ is primarily located in the cell membrane, where positioning is crucial for function in catalyzing the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a key signaling lipid involved in retrograde synaptic signaling and axonal growth. DAGLβ′s membrane localization allows effective interaction with substrate, facilitating rapid signaling responses in neurons. Additionally, DAGLβ operates optimally at a pH of 7 and is sensitive to inhibition by compounds such as p-hydroxy-mercuri-benzoate and mercury chloride, but not by phenylmethylsulfonyl fluoride. Alternative splicing of the DAGLβ gene results in three distinct isoforms, each potentially contributing to diverse regulatory mechanisms of lipid signaling pathways in the nervous system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DAGLβ Antibody (A-5) References:
- Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. | Nakajima, D., et al. 2002. DNA Res. 9: 99-106. PMID: 12168954
- Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. | Bisogno, T., et al. 2003. J Cell Biol. 163: 463-8. PMID: 14610053
- Stimulation of endocannabinoid formation in brain slice cultures through activation of group I metabotropic glutamate receptors. | Jung, KM., et al. 2005. Mol Pharmacol. 68: 1196-202. PMID: 16051747
- Endocannabinoid metabolic pathways and enzymes. | Ligresti, A., et al. 2005. Curr Drug Targets CNS Neurol Disord. 4: 615-23. PMID: 16375679
- Critical enzymes involved in endocannabinoid metabolism. | Basavarajappa, BS. 2007. Protein Pept Lett. 14: 237-46. PMID: 17346227
- Endocannabinoids and synaptic function in the CNS. | Hashimotodani, Y., et al. 2007. Neuroscientist. 13: 127-37. PMID: 17404373
- A duplication at chromosome 11q12.2-11q12.3 is associated with spinocerebellar ataxia type 20. | Knight, MA., et al. 2008. Hum Mol Genet. 17: 3847-53. PMID: 18801880
- Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. | Ishikawa, K., et al. 1998. DNA Res. 5: 169-76. PMID: 9734811
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAGLβ Antibody (A-5) | sc-514738 | 200 µg/ml | $322.00 | |||
DAGLβ Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-527936 | 200 µg Ab; 10 µg BP | $361.00 | |||
DAGLβ Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-533309 | 200 µg Ab; 20 µg BP | $361.00 | |||
DAGLβ Antibody (A-5) AC | sc-514738 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DAGLβ Antibody (A-5) HRP | sc-514738 HRP | 200 µg/ml | $322.00 | |||
DAGLβ Antibody (A-5) FITC | sc-514738 FITC | 200 µg/ml | $336.00 | |||
DAGLβ Antibody (A-5) PE | sc-514738 PE | 200 µg/ml | $349.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 488 | sc-514738 AF488 | 200 µg/ml | $364.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 546 | sc-514738 AF546 | 200 µg/ml | $364.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 594 | sc-514738 AF594 | 200 µg/ml | $364.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 647 | sc-514738 AF647 | 200 µg/ml | $364.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 680 | sc-514738 AF680 | 200 µg/ml | $364.00 | |||
DAGLβ Antibody (A-5) Alexa Fluor® 790 | sc-514738 AF790 | 200 µg/ml | $364.00 | |||
DAGLβ (A-5) Neutralizing Peptide | sc-514738 P | 100 µg/0.5 ml | $69.00 |