Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dab2 Double Nickase Plasmid (m): sc-419936-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dab2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dab2 Double Nickase Plasmid (m) and Dab2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dab2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dab2 Antibody (E-11): sc-136964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dab2 Double Nickase Plasmid (m) | sc-419936-NIC | 20 µg | $410.00 | |||
Dab2 Double Nickase Plasmid (m2) | sc-419936-NIC-2 | 20 µg | $410.00 |
Disabled homolog 2 (Dab2) encodes an endocytic adaptor protein that links cargo receptors to the clathrin-mediated internalization machinery through interactions with clathrin, AP-2, and phospholipids. In mouse cells, DAB2 participates in receptor trafficking and signal attenuation, influencing pathways such as TGF-β and Wnt/β-catenin by modulating receptor availability and downstream signaling intensity. It also contributes to epithelial polarity, cell adhesion, and cytoskeletal organization, processes central to developmental patterning and tissue homeostasis. Dysregulated DAB2 expression or function has been associated with altered differentiation and migratory phenotypes that are relevant to cancer biology and fibrotic or inflammatory remodeling in experimental models.
Dab2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dab2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dab2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dab2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dab2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.