Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dab2 Double Nickase Plasmid (h): sc-400979-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dab2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dab2 Double Nickase Plasmid (h) and Dab2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dab2 Antibody (E-11): sc-136964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dab2 Double Nickase Plasmid (h) | sc-400979-NIC | 20 µg | $410.00 | |||
Dab2 Double Nickase Plasmid (h2) | sc-400979-NIC-2 | 20 µg | $410.00 |
DAB2 (Disabled homolog 2) encodes Dab2, an endocytic adaptor protein that links clathrin-mediated internalization to signaling control by binding receptors and phosphoinositides through its PTB domain. Dab2 participates in receptor trafficking and attenuation of pathways such as TGF-β, Wnt/β-catenin, and MAPK/ERK, helping regulate cell polarity, adhesion, and differentiation programs. Through its roles in membrane trafficking and signal integration, altered DAB2 expression or function is associated with dysregulated epithelial homeostasis and has been studied in the context of oncogenic progression and metastasis-related phenotypes. These features make DAB2 a useful target for mechanistic studies of receptor turnover, pathway crosstalk, and endocytosis-driven control of cellular behavior.
Dab2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.