Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dab1 Double Nickase Plasmid (h): sc-402167-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dab1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dab1 Double Nickase Plasmid (h) and Dab1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dab1 Antibody (G-5): sc-271136
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dab1 Double Nickase Plasmid (h) | sc-402167-NIC | 20 µg | $410.00 | |||
Dab1 Double Nickase Plasmid (h2) | sc-402167-NIC-2 | 20 µg | $410.00 |
DAB1 encodes Disabled-1 (Dab1), an intracellular adaptor protein that relays signals from the Reelin pathway to coordinate neuronal migration, cortical lamination, and neurite outgrowth during human brain development. Upon receptor engagement, Dab1 undergoes tyrosine phosphorylation and scaffolds downstream effectors in Src family kinase, PI3K–AKT, and cytoskeletal remodeling processes that regulate cell positioning and polarity. DAB1 activity intersects with endocytic trafficking and microtubule/actin dynamics, linking extracellular cues to changes in adhesion and motility. Dysregulation of Reelin–DAB1 signaling has been associated with neurodevelopmental and neuropsychiatric phenotypes, making DAB1 a useful target for mechanistic studies of neuronal circuit formation.
Dab1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.