Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DAAO Double Nickase Plasmid (h): sc-402772-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAAO Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DAAO Double Nickase Plasmid (h) and DAAO Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAO. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAAO Antibody (B-3): sc-398757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAAO Double Nickase Plasmid (h) | sc-402772-NIC | 20 µg | $410.00 | |||
DAAO Double Nickase Plasmid (h2) | sc-402772-NIC-2 | 20 µg | $410.00 |
Human DAO encodes D-amino acid oxidase (DAAO), a peroxisomal flavoprotein that catalyzes oxidative deamination of neutral D-amino acids to the corresponding keto acids while generating hydrogen peroxide. This activity interfaces with amino acid catabolism, redox homeostasis, and peroxisome-associated reactive oxygen species metabolism, with particular relevance to regulation of D-serine availability and NMDA receptor co-agonism in the central nervous system. Altered DAO/DAAO function has been investigated in the context of neuropsychiatric and neurodegenerative disease biology, where shifts in D-serine turnover and oxidative stress pathways can influence synaptic signaling. DAO is also used as a metabolic node for studying peroxisomal enzyme trafficking, flavin-dependent oxidation chemistry, and cellular responses to peroxide.
DAAO Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAO locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAO. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAO function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAO-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.