Cytokeratin 8 Antibody (H-11) recommended for detection of Cytokeratin 8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cytokeratin 8 Antibody (H-11): sc-374027

Cytokeratin 8 Antibody (H-11) is rated 5.0 out of 5 by 4.
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Datasheets

    • Cytokeratin 8 Antibody (H-11) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 11-50 mapping near the N-terminus of Cytokeratin 8 of human origin
    • recommended for detection of Cytokeratin 8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Cytokeratin 8 (C51): sc-8020 for Cytokeratin 8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can Cytokeratin 8 (H-11): sc-374027 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Cytokeratin 8 (H-11): sc-374027 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HepG2. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Works well for WB with Hep G2 Works well for WB with Hep G2, MCF7 and T24 whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Good quality antibody for human paraffin samples Good quality antibody for human paraffin samples. Specific and sensitive -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using A-431 whole cell lysate. -SCBT QC
Date published: 2013-07-28
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