Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 18 CRISPR/Cas9 KO Plasmid (h): sc-400305
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cytokeratin 18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 18 Antibody (RGE53): sc-32329
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 18 CRISPR/Cas9 KO Plasmid (h) | sc-400305 | 20 µg | $397.00 |
Overview
KRT18 encodes cytokeratin 18, a type I intermediate filament protein that heteropolymerizes with keratin 8 to form the core cytoskeletal network of simple epithelial cells. This filament system supports cell architecture and mechanical resilience, and it interfaces with junctional complexes, organelle positioning, and stress-response signaling that coordinates cell cycle progression and apoptosis. Cytokeratin 18 dynamics are regulated by phosphorylation and proteolytic processing, linking it to cytoskeletal remodeling during mitosis and cellular stress. Dysregulated KRT18 expression or filament organization is frequently used as a marker of epithelial state and has been studied in contexts of epithelial injury, inflammation, and tumor cell biology.
Cytokeratin 18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KRT18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KRT18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KRT18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cytokeratin 18 protein expression.
This CRISPR knockout system enables efficient generation of KRT18-deficient cell models for investigation of Cytokeratin 18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KRT18 exon(s) critical for Cytokeratin 18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KRT18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cytokeratin 18 CRISPR/Cas9 KO Plasmid (h) and Cytokeratin 18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KRT18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cytokeratin 18 HDR Plasmid (h) and Cytokeratin 18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KRT18 homology arms to support homology-directed repair at defined KRT18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.