Date published: 2026-5-21

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Cytokeratin 18 Antibody (B-6): sc-398871

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Datasheets
  • Cytokeratin 18 Antibody (B-6) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-80 mapping at the N-terminus of Cytokeratin 18 of human origin
  • recommended for detection of Cytokeratin 18 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See Cytokeratin 18 (RGE53): sc-32329 for Cytokeratin 18 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cytokeratin 18 Antibody (B-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 18 Antibody (B-6) (see ordering information below).
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Cytokeratin 18 Antibody (B-6) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 18 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Cytokeratin 18 is a vital component of the cytoskeleton in epithelial cells, contributing significantly to structural integrity and resilience. Cytokeratin 18 is predominantly located in the cytoplasm, forming a network of intermediate filaments that provide mechanical support and shape to cells. Proper localization of Cytokeratin 18 is crucial for maintaining epithelial tissue architecture, and disruption can lead to various pathological conditions, including cancer. Cytokeratin 18 serves as a biomarker for epithelial-derived tumors, as expression levels can indicate differentiation status of malignant cells. Post-translational modifications of Cytokeratin 18, such as phosphorylation, can influence protein interactions and overall cellular function. Anti-Cytokeratin 18 antibody (B-6) serves as an essential tool for researchers studying epithelial biology and cancer pathology, providing insights into Cytokeratin 18′s role in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cytokeratin 18 Antibody (B-6)

sc-398871
200 µg/ml
$322.00

Cytokeratin 18 Antibody (B-6): m-IgG Fc BP-HRP Bundle

sc-540951
200 µg Ab; 10 µg BP
$361.00

Cytokeratin 18 Antibody (B-6): m-IgG1 BP-HRP Bundle

sc-542484
200 µg Ab; 20 µg BP
$361.00

sc-398871 B-6を凍結切片の染色で使用しています。今まではヒト切片でしたが、マウス切片も使用することとなりました。B-6はconjucated Abはないと思うのですが、どのように染色するのがおすすめでしょうか。 どうぞよろしくお願いいたします。

Asked by: cocoa1107milk
Thank you for your question. Please contact our Technical Service Department for more details on this question. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-07-10

Can Cytokeratin 18 (B-6): sc-398871 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, Cytokeratin 18 (B-6): sc-398871 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great Western blot dataGreat Western blot data! Works in MCF7, HeLa, A-431, Hep G2 and ECV304 whole cell lysates. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Beautiful IF data in methanolBeautiful IF data in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-19
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Cytokeratin 18 Antibody (B-6) is rated 5.0 out of 5 by 2.
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