Cytokeratin 18 Antibody (2X44) recommended for detection of Cytokeratin 18 of human origin by WB, IP, IF, IHC(P) and FCM

Cytokeratin 18 Antibody (2X44): sc-70917

Cytokeratin 18 Antibody (2X44) is rated 4.0 out of 5 by 3.
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Datasheets

    • Cytokeratin 18 Antibody (2X44) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
    • raised against cytokeratins from the human bladder carcinoma cell line T24
    • recommended for detection of Cytokeratin 18 of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Cytokeratin 18 (RGE53): sc-32329 for Cytokeratin 18 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can Cytokeratin 18 (2X44): sc-70917 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, Cytokeratin 18 (2X44): sc-70917 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Single strong band in MCF7 whole cell lysates Single strong band in MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 3 out of 5 by from Worked with human transfected lysate by WB Worked with human transfected lysate by WB -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by from Positive WB data in Hep G2 Positive WB data in Hep G2. -SCBT QC
Date published: 2015-01-02
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