Cytokeratin 16 Antibody (LL025): sc-53255

Cytokeratin 16 Antibody (LL025) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cytokeratin 16 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Cytokeratin 16 antibody (LL025) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 16 is a member of the cytokeratin family, which consists of a diverse group of intermediate filament proteins crucial for maintaining the structural integrity of epithelial cells. Cytokeratin 16 (LL025) monoclonal antibody primarily targets proteins in the cytoplasm of epithelial cells, particularly in stratified squamous epithelium, where Cytokeratin 16 plays a vital role in providing mechanical support and resilience against physical stress. Cytokeratin 16 serves as a marker for identifying tumor origin in squamous cell carcinoma and can indicate tumor differentiation status. Expression of Cytokeratin 16 occurs in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as in luminal cells of the mammary gland and sweat ducts, while remaining absent in noninvasive breast carcinomas and normal breast tissue. Mutations in Cytokeratin 16 are associated with several skin disorders, including pachyonychia congenita type 1, nonepidermolytic palmoplantar keratoderma, and unilateral palmoplantar verrucous nevus, highlighting Cytokeratin 16′s significance in both normal physiology and disease states.

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Cytokeratin 16 Antibody (LL025) References:

1. [Clinicopathological and immunohistochemical study of oral teratoid cyst].  |  Shen, J., et al. 2005. Zhonghua Kou Qiang Yi Xue Za Zhi. 40: 62-6. PMID: 15774156
2. Clinicopathological and immunohistochemical study of 39 cases of Adenomatoid Odontogenic Tumour: a multicentric study.  |  Leon, JE., et al. 2005. Oral Oncol. 41: 835-42. PMID: 15979930
3. Cytokeratins 6 and 16 are frequently expressed in head and neck squamous cell carcinoma cell lines and fresh biopsies.  |  Sesterhenn, AM., et al. 2005. Anticancer Res. 25: 2675-80. PMID: 16080511
4. Basal cell-specific and hyperproliferation-related keratins in human breast cancer.  |  Wetzels, RH., et al. 1991. Am J Pathol. 138: 751-63. PMID: 1705754

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 16 Antibody (LL025) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 16 Antibody (LL025) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 16 Antibody (LL025) Specifications

Alternate Product Name: KRT16

Product Clone Name: LL025

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cytokeratin 16

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 16

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 16 Antibody (LL025): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin