Cytokeratin 15 Double Nickase Plasmid (h): sc-401869-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cytokeratin 15 Double Nickase Plasmid (h) and Cytokeratin 15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KRT15. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 15 Antibody (LHK15): sc-47697
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 15 Double Nickase Plasmid (h) | sc-401869-NIC | 20 µg | $410.00 | |||
Cytokeratin 15 Double Nickase Plasmid (h2) | sc-401869-NIC-2 | 20 µg | $410.00 |
KRT15 encodes cytokeratin 15, a type I intermediate filament protein enriched in basal keratinocytes and epithelial progenitor compartments where it contributes to cytoskeletal integrity and tissue resilience. Cytokeratin 15 participates in intermediate filament assembly and cross-talks with desmosomal and hemidesmosomal adhesion complexes, influencing cell–cell adhesion, polarity, and migration programs. In skin and stratified epithelia, KRT15 expression is commonly used to study stem cell dynamics, epidermal differentiation, and wound repair processes. Dysregulated keratin networks, including altered KRT15 expression patterns, are associated with epithelial stress responses and have been investigated in contexts of hyperproliferation, inflammation, and tumor biology.
Cytokeratin 15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KRT15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KRT15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KRT15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KRT15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.