Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 10 Double Nickase Plasmid (h): sc-400193-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cytokeratin 10 Double Nickase Plasmid (h) and Cytokeratin 10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KRT10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 10 Antibody (LH2): sc-53252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 10 Double Nickase Plasmid (h) | sc-400193-NIC | 20 µg | $410.00 | |||
Cytokeratin 10 Double Nickase Plasmid (h2) | sc-400193-NIC-2 | 20 µg | $410.00 |
KRT10 encodes cytokeratin 10, a type I intermediate filament protein that pairs with keratin 1 to form the suprabasal keratin network in stratified epithelia. This filament system provides mechanical resilience, supports desmosome-associated cell–cell adhesion, and contributes to terminal differentiation and epidermal barrier formation. KRT10 expression is tightly linked to keratinocyte maturation programs and cross-talk with calcium-dependent differentiation and stress-response pathways that remodel the cytoskeleton. Genetic disruption or dysregulated expression of KRT10 is associated with inherited disorders of cornification, including epidermolytic ichthyosis, and is frequently used as a marker of altered epithelial differentiation in dermatologic disease models.
Cytokeratin 10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KRT10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KRT10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KRT10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KRT10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.