cytohesin-2 Antibody (CYT2-21): sc-59451

Cytohesin-2 Antibody (CYT2-21) is a mouse monoclonal IgG1 antibody that detects cytohesin-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cytohesin-2, also known as ARNO (ARF nucleotide-binding-site opener), functions as a guanine nucleotide exchange factor (GEF) for ARF-1, playing a crucial role in vesicle budding, which is essential for intracellular transport and communication. Cytohesin-2′s structure includes an N-terminal coiled-coil domain, a Sec 7 domain that facilitates GDP/GTP exchange, and a C-terminal pleckstrin homology (PH) domain that binds to phosphatidylinositol 4,5-bisphosphate (PIP2). This binding enhances cytohesin-2′s GEF activity by recruiting cytohesin-2 to the plasma membrane, where cytohesin-2 can effectively stimulate nucleotide exchange on ARF1 and ARF6. Localization of cytohesin-2 to the plasma membrane in mammalian cells underscores cytohesin-2′s importance in regulating membrane dynamics and vesicle trafficking, making cytohesin-2 a vital component in cellular signaling pathways. Anti-cytohesin-2 antibody (CYT2-21) serves as an invaluable tool for researchers studying these processes and their implications in various biological contexts.

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cytohesin-2 Antibody (CYT2-21) References:

1. Interaction protein for cytohesin exchange factors 1 (IPCEF1) binds cytohesin 2 and modifies its activity.  |  Venkateswarlu, K. 2003. J Biol Chem. 278: 43460-9. PMID: 12920129
2. Cytohesin-2/ARNO, through its interaction with focal adhesion adaptor protein paxillin, regulates preadipocyte migration via the downstream activation of Arf6.  |  Torii, T., et al. 2010. J Biol Chem. 285: 24270-81. PMID: 20525696
3. Cytohesin-2 phosphorylation by protein kinase C relieves the constitutive suppression of platelet dense granule secretion by ADP-ribosylation factor 6.  |  van den Bosch, MT., et al. 2014. J Thromb Haemost. 12: 726-35. PMID: 24581425
4. Cytohesin 2/ARF6 regulates preadipocyte migration through the activation of ERK1/2.  |  Davies, JC., et al. 2014. Biochem Pharmacol. 92: 651-60. PMID: 25450674
5. Arf6 guanine-nucleotide exchange factor cytohesin-2 regulates myelination in nerves.  |  Torii, T., et al. 2015. Biochem Biophys Res Commun. 460: 819-25. PMID: 25824033
6. ARF-GEF cytohesin-2/ARNO regulates R-Ras and α5-integrin recycling through an EHD1-positive compartment.  |  Salem, JC., et al. 2015. Mol Biol Cell. 26: 4265-79. PMID: 26378252
7. Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.  |  Ito, A., et al. 2021. Neurobiol Dis. 159: 105466. PMID: 34390832
8. The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice.  |  Miyamoto, Y., et al. 2022. Sci Signal. 15: eabi5276. PMID: 35077201
9. Cytohesin-2/ARNO: A Novel Bridge Between Cell Migration and Immunoregulation in Synovial Fibroblasts.  |  Wang, Y., et al. 2021. Front Immunol. 12: 809896. PMID: 35095899
10. Blocking the cytohesin-2/ARF1 axis by SecinH3 ameliorates osteoclast-induced bone loss via attenuating JNK-mediated IRE1 endoribonuclease activity.  |  Dong, Y., et al. 2022. Pharmacol Res. 185: 106513. PMID: 36252772

The preferred cytohesin-2 antibody is cytohesin-2 Antibody (H-7): sc-374640.