cytohesin-2 Antibody (H-7): sc-374640
- cytohesin-2 Antibody (H-7) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 31-67 mapping near the N-terminus of cytohesin-2 of human origin
- Anti-cytohesin-2 Antibody (H-7) is recommended for detection of cytohesin-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-cytohesin-2 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for cytohesin-2 Antibody (H-7). This work is in progress.
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Cytohesin-2 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects cytohesin-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cytohesin-2 antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytohesin-2, also known as ARNO (ARF nucleotide-binding-site opener), functions as a guanine nucleotide exchange factor (GEF) for ARF1 and ARF6, which are critical in regulating vesicle budding and trafficking within cells. Cytohesin-2′s structure includes an N-terminal coiled-coil domain, a Sec 7 domain that facilitates GDP/GTP exchange, and a C-terminal pleckstrin homology (PH) domain that binds to phosphatidylinositol 4,5-bisphosphate (PIP2). This binding enhances cytohesin-2′s GEF activity by recruiting cytohesin-2 to the plasma membrane, where cytohesin-2 can effectively stimulate nucleotide exchange on ARF proteins. Plasma membrane localization of cytohesin-2 ensures optimal positioning for substrate interaction and participation in membrane dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cytohesin-2 Antibody (H-7) References:
- Cytohesin-2 as a novel prognostic marker for hepatocellular carcinoma. | Xu, K., et al. 2013. Oncol Rep. 29: 2211-8. PMID: 23545718
- Arf6 guanine nucleotide exchange factor cytohesin-2 binds to CCDC120 and is transported along neurites to mediate neurite growth. | Torii, T., et al. 2014. J Biol Chem. 289: 33887-903. PMID: 25326380
- Pallidin is a novel interacting protein for cytohesin-2 and regulates the early endosomal pathway and dendritic formation in neurons. | Ito, A., et al. 2018. J Neurochem. 147: 153-177. PMID: 30151872
- The GEF Cytohesin-2/ARNO Mediates Resistin induced Phenotypic Switching in Vascular Smooth Muscle Cells. | Heun, Y., et al. 2020. Sci Rep. 10: 3672. PMID: 32111889
- Cytohesin-2 Is Upregulated in Malignant Melanoma and Contributes to Tumor Growth. | Lee, YJ., et al. 2019. Ann Dermatol. 31: 93-96. PMID: 33911549
- S100A6, Calumenin and Cytohesin 2 as Biomarkers for Cutaneous Involvement in Systemic Sclerosis Patients: A Case Control Study. | Balanescu, P., et al. 2021. J Pers Med. 11: PMID: 34063287
- Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin. | Kahn, RA. and Gilman, AG. 1984. J Biol Chem. 259: 6228-34. PMID: 6327671
- Structure and function of ARF proteins: activators of cholera toxin and critical components of intracellular vesicular transport processes. | Moss, J. and Vaughan, M. 1995. J Biol Chem. 270: 12327-30. PMID: 7759471
- A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains. | Chardin, P., et al. 1996. Nature. 384: 481-4. PMID: 8945478
- Role of protein-phospholipid interactions in the activation of ARF1 by the guanine nucleotide exchange factor Arno. | Paris, S., et al. 1997. J Biol Chem. 272: 22221-6. PMID: 9268368
- ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6. | Frank, S., et al. 1998. J Biol Chem. 273: 23-7. PMID: 9417041
- ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function. | Franco, M., et al. 1998. Proc Natl Acad Sci U S A. 95: 9926-31. PMID: 9707577
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cytohesin-2 Antibody (H-7) | sc-374640 | 200 µg/ml | $316.00 | |||
cytohesin-2 Antibody (H-7) AC | sc-374640 AC | 500 µg/ml, 25% agarose | $416.00 | |||
cytohesin-2 Antibody (H-7) HRP | sc-374640 HRP | 200 µg/ml | $316.00 | |||
cytohesin-2 Antibody (H-7) FITC | sc-374640 FITC | 200 µg/ml | $330.00 | |||
cytohesin-2 Antibody (H-7) PE | sc-374640 PE | 200 µg/ml | $343.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 488 | sc-374640 AF488 | 200 µg/ml | $357.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 546 | sc-374640 AF546 | 200 µg/ml | $357.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 594 | sc-374640 AF594 | 200 µg/ml | $357.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 647 | sc-374640 AF647 | 200 µg/ml | $357.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 680 | sc-374640 AF680 | 200 µg/ml | $357.00 | |||
cytohesin-2 Antibody (H-7) Alexa Fluor® 790 | sc-374640 AF790 | 200 µg/ml | $357.00 |