cytohesin-1 Antibody (CYT1-82): sc-59491

Cytohesin-1 Antibody (CYT1-82) is a mouse monoclonal IgG1 antibody that detects cytohesin-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cytohesin-1 antibody (CYT1-82) is available as a non-conjugated format. Cytohesin-1 functions as an ARF guanine nucleotide exchange factor (GEF), playing a crucial role in regulating intracellular trafficking and signal transduction. Cytohesin-1 is primarily located in the cytoplasm and translocates to the plasma membrane upon activation, where cytohesin-1 interacts with phospholipids and other proteins to facilitate membrane dynamics. A phospholipid-binding C-terminal pleckstrin homology (PH) domain, central Sec7 domain, and N-terminal coiled-coil region in cytohesin-1′s structure are essential for function, as the PH domain specifically binds to phosphatidylinositol 3,4,5-triphosphate (PtdIns-3,4,5-P3), a key signaling lipid. The Sec7 domain is critical for GDP/GTP exchange activity, which is vital for ARF protein activation, while the coiled-coil region may mediate protein-protein interactions. Cytohesin-1 also serves as a regulatory factor for αLβ2 integrin in lymphocytes, suggesting involvement in inside-out signaling pathways that modulate cell adhesion and migration. Cytohesin-1′s ability to catalyze nucleotide exchange on ARF1 and ARF3, while being unaffected by ARF6, highlights specificity and importance in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cytohesin-1 Antibody (CYT1-82) References:

1. Cloning and sequencing of a human cDNA from cytolytic NK/T cells with homology to yeast SEC7.  |  Liu, L. and Pohajdak, B. 1992. Biochim Biophys Acta. 1132: 75-8. PMID: 1511013
2. Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule.  |  Kolanus, W., et al. 1996. Cell. 86: 233-42. PMID: 8706128
3. Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor.  |  Meacci, E., et al. 1997. Proc Natl Acad Sci U S A. 94: 1745-8. PMID: 9050849
4. Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains.  |  Klarlund, JK., et al. 1997. Science. 275: 1927-30. PMID: 9072969
5. Brefeldin A-inhibited guanine nucleotide-exchange activity of Sec7 domain from yeast Sec7 with yeast and mammalian ADP ribosylation factors.  |  Sata, M., et al. 1998. Proc Natl Acad Sci U S A. 95: 4204-8. PMID: 9539714
6. ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function.  |  Franco, M., et al. 1998. Proc Natl Acad Sci U S A. 95: 9926-31. PMID: 9707577

The preferred cytohesin-1/2 antibody is cytohesin-1/2 Antibody (D-11): sc-166542.