cytochrome c1 Antibody (A-5): sc-514435
- cytochrome c1 Antibody (A-5) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 150-170 within an internal region of cytochrome c1 of human origin
- Anti-cytochrome c1 Antibody (A-5) is recommended for detection of cytochrome c1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cytochrome c1 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cytochrome c1 Antibody (A-5) for WB applications. These reagents are now offered in bundles with cytochrome c1 Antibody (A-5) (see ordering information below).
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cytochrome c1 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects cytochrome c1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-cytochrome c1 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cytochrome c1 plays a crucial role as a component of the ubiquinol-cytochrome c reductase complex, which is integral to the mitochondrial respiratory chain, facilitating the transfer of electrons and contributing to the generation of an electrochemical gradient necessary for ATP synthesis. cytochrome c1 is located within the inner mitochondrial membrane, where proper function is vital for cellular respiration and energy production. cytochrome c1 interaction with other subunits of the bc1 complex, including cytochrome b and the Rieske protein, is essential for the efficient transfer of electrons, highlighting importance in maintaining cellular energy homeostasis. cytochrome c1 gene regulation by transcription factors such as E2F and Sp1 underscores the complexity of expression and potential implications for metabolic regulation in various physiological and pathological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cytochrome c1 Antibody (A-5) References:
- Activity of the human cytochrome c1 promoter is modulated by E2F. | Luciakova, K., et al. 2000. Biochem J. 351: 251-6. PMID: 10998368
- Mitochondrial cytochrome c1 is a collapsed di-heme cytochrome. | Baymann, F., et al. 2004. Proc Natl Acad Sci U S A. 101: 17737-40. PMID: 15591339
- A structural analysis of the transient interaction between the cytochrome bc1 complex and its substrate cytochrome c. | Nyola, A. and Hunte, C. 2008. Biochem Soc Trans. 36: 981-5. PMID: 18793174
- Common protein-binding sites in the 5'-flanking regions of human genes for cytochrome c1 and ubiquinone-binding protein. | Suzuki, H., et al. 1990. J Biol Chem. 265: 8159-63. PMID: 2159470
- Isolation of a cDNA clone for human cytochrome c1 from a lambda gt11 expression library. | Nishikimi, M., et al. 1987. Biochem Biophys Res Commun. 145: 34-9. PMID: 3036122
- Assignment of the gene for the cytochrome c1 subunit of the mitochondrial cytochrome bc1 complex (CYC1) to human chromosome 8q24.3. | Duncan, AM., et al. 1994. Genomics. 19: 400-1. PMID: 8188279
- Expression of the human cytochrome c1 gene is controlled through multiple Sp1-binding sites and an initiator region. | Li, R., et al. 1996. Eur J Biochem. 241: 649-56. PMID: 8917468
- Electron transfer by domain movement in cytochrome bc1. | Zhang, Z., et al. 1998. Nature. 392: 677-84. PMID: 9565029
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cytochrome c1 Antibody (A-5) | sc-514435 | 200 µg/ml | $316.00 | |||
cytochrome c1 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-527901 | 200 µg Ab; 10 µg BP | $354.00 | |||
cytochrome c1 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-533274 | 200 µg Ab; 20 µg BP | $354.00 | |||
cytochrome c1 Antibody (A-5) AC | sc-514435 AC | 500 µg/ml, 25% agarose | $416.00 | |||
cytochrome c1 Antibody (A-5) HRP | sc-514435 HRP | 200 µg/ml | $316.00 | |||
cytochrome c1 Antibody (A-5) FITC | sc-514435 FITC | 200 µg/ml | $330.00 | |||
cytochrome c1 Antibody (A-5) PE | sc-514435 PE | 200 µg/ml | $343.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 488 | sc-514435 AF488 | 200 µg/ml | $357.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 546 | sc-514435 AF546 | 200 µg/ml | $357.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 594 | sc-514435 AF594 | 200 µg/ml | $357.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 647 | sc-514435 AF647 | 200 µg/ml | $357.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 680 | sc-514435 AF680 | 200 µg/ml | $357.00 | |||
cytochrome c1 Antibody (A-5) Alexa Fluor® 790 | sc-514435 AF790 | 200 µg/ml | $357.00 | |||
cytochrome c1 (A-5) Neutralizing Peptide | sc-514435 P | 100 µg/0.5 ml | $68.00 |