cytochrome c Antibody (6H2): sc-13561

Cytochrome c Antibody (6H2) is a mouse monoclonal IgG1 kappa light chain antibody raised against full-length rat cytochrome c, reacting with mouse, rat, and human proteins. Cytochrome c, also known as CYCS, is a small heme protein located in the mitochondrial intermembrane space and plays a pivotal role in the electron transport chain of oxidative phosphorylation by transferring electrons between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase). This electron transfer is essential for ATP production in aerobic organisms, making cytochrome c vital for cellular energy metabolism. Upon release into the cytosol, cytochrome c interacts with apoptotic protease activating factor-1 (Apaf-1) to form the apoptosome, leading to the activation of caspase-9 and downstream effector caspases like caspase-3. Release of cytochrome c is regulated by Bcl-2 family proteins, such as Bcl-2, which inhibits release, and Bax, which promotes release, highlighting importance in cell death regulation. Interactions with these proteins are crucial for understanding mechanisms underlying apoptosis and related diseases like cancer and neurodegenerative disorders. Cytochrome c monoclonal antibody (6H2) is suitable for applications including western blotting, immunoprecipitation, flow cytometry (FCM), and ELISA, providing a valuable tool for researchers studying mitochondrial function, energy metabolism, and apoptotic pathways. Cytochrome c (6H2) antibody aids in exploring protein interactions and roles in cellular processes essential for life and disease progression.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cytochrome c Antibody (6H2) has 162 citations in a variety of scientific publications.

Primary antibodies like Anti-cytochrome c Antibody (6H2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cytochrome c Antibody (6H2) Specifications

Alternate Product Name: cytochrome c

Product Clone Name: 6H2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: cytochrome c

Epitope Species: rat

Product Reactivity/Detection: native form of cytochrome c

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 162 citations in various scientific publications

Available Conjugates/Forms for cytochrome c Antibody (6H2): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred cytochrome c antibody is cytochrome c Antibody (A-8): sc-13156.