CYTIP Antibody (H-6): sc-390857
- CYTIP Antibody (H-6) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 183-345 mapping near the C-terminus of CYTIP of mouse origin
- Anti-CYTIP Antibody (H-6) is recommended for detection of CYTIP of mouse and rat origin by WB, IP, IF and ELISA
- Anti-CYTIP Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CYTIP Antibody (H-6) for WB applications. These reagents are now offered in bundles with CYTIP Antibody (H-6) (see ordering information below).
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CYTIP Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYTIP in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYTIP Antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYTIP, also known as PSCDBP (pleckstrin homology, Sec7 and coiled-coil domains, binding protein), CASP, or CYBR, is a cytoplasmic protein primarily expressed in hematopoietic cells, playing a crucial role in lymphocytic cell adhesion. CYTIP regulates cytohesin-1 activity, an integrin-activating protein essential for cell adhesion, by mediating recruitment to the leukocyte membrane. Through binding to cytohesin-1, CYTIP sequesters cytohesin-1 in the cytoplasm, preventing translocation to lymphocytes and disrupting cell adhesion signaling pathways. CYTIP can be recruited to the membrane by leukocyte integrins that interact with PDZ domain, and returns to the cytoplasm upon exposure to phorbol esters. CYTIP associates with sorting nexin 27, which aids in coordinating trafficking and signaling complexes. Up-regulation of CYTIP in maturing dendritic cells indicates a potential role in developmentally-controlled cell adhesion, making CYTIP a significant target for research in immunology and cell biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYTIP Antibody (H-6) References:
- Assignment of the human B3-1 gene (PSCDBP) to chromosome 2 band q11.2 by radiation hybrid mapping. | Kim, HS. 1999. Cytogenet Cell Genet. 84: 95. PMID: 10343115
- Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity. | Tang, P., et al. 2002. Proc Natl Acad Sci U S A. 99: 2625-9. PMID: 11867758
- Attenuation of cell adhesion in lymphocytes is regulated by CYTIP, a protein which mediates signal complex sequestration. | Boehm, T., et al. 2003. EMBO J. 22: 1014-24. PMID: 12606567
- Dendritic cells regulate T-cell deattachment through the integrin-interacting protein CYTIP. | Hofer, S., et al. 2006. Blood. 107: 1003-9. PMID: 16204317
- Cytohesin binder and regulator augments T cell receptor-induced nuclear factor of activated T Cells.AP-1 activation through regulation of the JNK pathway. | Chen, Q., et al. 2006. J Biol Chem. 281: 19985-94. PMID: 16702224
- Cytohesin binder and regulator (cybr) is not essential for T- and dendritic-cell activation and differentiation. | Watford, WT., et al. 2006. Mol Cell Biol. 26: 6623-32. PMID: 16914744
- Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes. | MacNeil, AJ., et al. 2007. Biochem Biophys Res Commun. 359: 848-53. PMID: 17577583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYTIP Antibody (H-6) | sc-390857 | 200 µg/ml | $322.00 | |||
CYTIP Antibody (H-6): m-IgG Fc BP-HRP Bundle | sc-526199 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYTIP Antibody (H-6): m-IgG2a BP-HRP Bundle | sc-546528 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYTIP Antibody (H-6) AC | sc-390857 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CYTIP Antibody (H-6) HRP | sc-390857 HRP | 200 µg/ml | $322.00 | |||
CYTIP Antibody (H-6) FITC | sc-390857 FITC | 200 µg/ml | $336.00 | |||
CYTIP Antibody (H-6) PE | sc-390857 PE | 200 µg/ml | $349.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 488 | sc-390857 AF488 | 200 µg/ml | $364.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 546 | sc-390857 AF546 | 200 µg/ml | $364.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 594 | sc-390857 AF594 | 200 µg/ml | $364.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 647 | sc-390857 AF647 | 200 µg/ml | $364.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 680 | sc-390857 AF680 | 200 µg/ml | $364.00 | |||
CYTIP Antibody (H-6) Alexa Fluor® 790 | sc-390857 AF790 | 200 µg/ml | $364.00 |