Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cystatin SA CRISPR/Cas9 KO Plasmid (h): sc-406907
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cystatin SA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cystatin SA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cystatin SA CRISPR/Cas9 KO Plasmid (h) | sc-406907 | 20 µg | $397.00 |
Overview
CST2 encodes cystatin SA, a secreted cysteine protease inhibitor that restrains papain-like cathepsins and related proteolytic activity at mucosal surfaces. By modulating protease–antiprotease balance, cystatin SA helps regulate extracellular matrix turnover, peptide processing, and inflammatory signaling within the oral and upper aerodigestive microenvironment. Altered expression of salivary cystatins has been associated with changes in innate defense, microbiome interactions, and proteolysis-linked tissue remodeling observed across inflammatory conditions and cancer-adjacent phenotypes. CST2 is therefore relevant for studying protease-driven pathways impacting epithelial barrier function and secretome composition.
cystatin SA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CST2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CST2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CST2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cystatin SA protein expression.
This CRISPR knockout system enables efficient generation of CST2-deficient cell models for investigation of cystatin SA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CST2 exon(s) critical for cystatin SA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CST2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cystatin SA CRISPR/Cas9 KO Plasmid (h) and cystatin SA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CST2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cystatin SA HDR Plasmid (h) and cystatin SA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CST2 homology arms to support homology-directed repair at defined CST2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.