cystatin A Antibody (B-11): sc-376759

cystatin A Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects cystatin A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-cystatin A antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cystatin A, also known as STF1, STFA, stefin A, or cystatin AS, is a crucial thiol protease inhibitor that plays a significant role in regulating proteolytic activity within cells. Cystatin A is primarily located in the cytoplasm and serves as one of the precursor proteins of the cornified cell envelope in keratinocytes, which is essential for epidermal development and maintenance. Proper functioning of cystatin A is vital for skin integrity, helping prevent excessive proteolysis that can lead to skin disorders. Cystatin A forms complexes with various proteases, including papain and cathepsins B, H, and L, thereby modulating their activity and contributing to the balance of proteolytic processes in the skin.

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cystatin A Antibody (B-11) References:

1. Expression of human cystatin A by keratinocytes is positively regulated via the Ras/MEKK1/MKK7/JNK signal transduction pathway but negatively regulated via the Ras/Raf-1/MEK1/ERK pathway.  |  Takahashi, H., et al. 2001. J Biol Chem. 276: 36632-8. PMID: 11451947
2. Different propensity to form amyloid fibrils by two homologous proteins-Human stefins A and B: searching for an explanation.  |  Jenko, S., et al. 2004. Proteins. 55: 417-25. PMID: 15048832
3. Cloning a synthetic gene for human stefin B and its expression in E. coli.  |  Jerala, R., et al. 1988. FEBS Lett. 239: 41-4. PMID: 3053245
4. Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver.  |  Ritonja, A., et al. 1985. Biochem Biophys Res Commun. 131: 1187-92. PMID: 3902020
5. Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1).  |  Pennacchio, LA., et al. 1996. Science. 271: 1731-4. PMID: 8596935
6. Structure and transcriptional regulation of the human cystatin A gene. The 12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element-2 site (-272 to -278) on cystatin A gene is critical for TPA-dependent regulation.  |  Takahashi, H., et al. 1998. J Biol Chem. 273: 17375-80. PMID: 9651321
7. Cysteine proteinases and their endogenous inhibitors: target proteins for prognosis, diagnosis and therapy in cancer (review).  |  Kos, J. and Lah, TT. 1998. Oncol Rep. 5: 1349-61. PMID: 9769367

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cystatin A Antibody (B-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-cystatin A Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cystatin A Antibody (B-11) Specifications

Alternate Product Name: cystatin A

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-98

Epitope Target: cystatin A

Epitope Species: human

Product Reactivity/Detection: cystatin A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for cystatin A Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin