CYP51A1 CRISPR/Cas9 KO Plasmid (m): sc-419931
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP51A1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CYP51A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP51A1 CRISPR/Cas9 KO Plasmid (m) | sc-419931 | 20 µg | $397.00 |
Overview
Mouse Cyp51 encodes the cytochrome P450 enzyme CYP51A1 (lanosterol 14α-demethylase), a conserved monooxygenase that catalyzes a key step in the post-squalene mevalonate/sterol biosynthesis pathway leading to cholesterol and other sterols. By controlling sterol intermediate flux, CYP51A1 influences membrane composition, lipid raft–dependent signaling, and the availability of sterol-derived metabolites that modulate cellular homeostasis. Perturbation of sterol biosynthesis is linked to altered proliferation, differentiation, and stress responses, making Cyp51 a useful node for studying metabolic rewiring and organ-specific lipid requirements. In mouse models, manipulating Cyp51 supports mechanistic investigation of cholesterol pathway regulation and its contribution to developmental and metabolic phenotypes.
CYP51A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cyp51 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cyp51 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cyp51 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CYP51A1 protein expression.
This CRISPR knockout system enables efficient generation of Cyp51-deficient cell models for investigation of CYP51A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cyp51 exon(s) critical for CYP51A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cyp51 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CYP51A1 CRISPR/Cas9 KO Plasmid (m) and CYP51A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cyp51 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CYP51A1 HDR Plasmid (m) and CYP51A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cyp51 homology arms to support homology-directed repair at defined Cyp51 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.