CYP46 Antibody (1F11): sc-136148
- CYP46 Antibody (1F11) is a mouse monoclonal IgG1 κ CYP46 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against partially purified recombinant CYP46 of human origin
- CYP46 Antibody (1F11) is recommended for detection of CYP46 of mouse, rat and human origin by WB and IF
- Anti-CYP46 Antibody (1F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CYP46 Antibody (1F11) for WB applications. These reagents are now offered in bundles with CYP46 Antibody (1F11) (see ordering information below).
CYP46 Antibody (1F11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP46 protein of mouse, rat, and human origin by western blotting (WB) and immunofluorescence (IF). Anti-CYP46 antibody (1F11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP46, also known as CYP46A1, is a crucial enzyme located in the endoplasmic reticulum, where CYP46 plays a vital role in cholesterol metabolism by converting cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. This conversion is essential for maintaining cholesterol homeostasis in the brain, as excess cholesterol can lead to neurodegenerative conditions. Notably, variations in the CYP46 gene that affect function have been linked to an increased risk of Alzheimer′s disease, highlighting CYP46′s significance in neurological health. With a sequence identity of 95% to its mouse counterpart, CYP46 is predominantly expressed in brain tissue, underscoring CYP46′s importance in central nervous system function and the regulation of cholesterol levels.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP46 Antibody (1F11) References:
- CYP46: a risk factor for Alzheimer's disease or a coincidence? | Golanska, E., et al. Neurosci Lett. 383: 105-8. PMID: 15936520
- A cluster of cholesterol-related genes confers susceptibility for Alzheimer's disease. | Papassotiropoulos, A., et al. 2005. J Clin Psychiatry. 66: 940-7. PMID: 16013913
- Polymorphisms of cholesterol metabolism genes CYP46 and ABCA1 and the risk of sporadic Alzheimer's disease in Chinese. | Wang, F. and Jia, J. 2007. Brain Res. 1147: 34-8. PMID: 17335784
- Meta-analysis of genetic variability in the beta-amyloid production, aggregation and degradation metabolic pathways and the risk of Alzheimer's disease. | Llorca, J., et al. 2008. Acta Neurol Scand. 117: 1-14. PMID: 17854420
- Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principal cholesterol hydroxylase in the brain. | Mast, N., et al. 2008. Proc Natl Acad Sci U S A. 105: 9546-51. PMID: 18621681
- Cholesterol 24-hydroxylase (CYP46A1) polymorphisms are associated with faster cognitive deterioration in Chinese older persons: a two-year follow up study. | Fu, BY., et al. 2009. Int J Geriatr Psychiatry. 24: 921-6. PMID: 19212968
- CYP46A1 variants influence Alzheimer's disease risk and brain cholesterol metabolism. | Kölsch, H., et al. 2009. Eur Psychiatry. 24: 183-90. PMID: 19286353
- Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation. | Björkhem, I., et al. 1998. J Lipid Res. 39: 1594-600. PMID: 9717719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP46 Antibody (1F11) | sc-136148 | 200 µg/ml | $316.00 | |||
CYP46 Antibody (1F11): m-IgG Fc BP-HRP Bundle | sc-527105 | 200 µg Ab; 10 µg BP | $354.00 | |||
CYP46 Antibody (1F11): m-IgG1 BP-HRP Bundle | sc-532478 | 200 µg Ab; 20 µg BP | $354.00 | |||
CYP46 Antibody (1F11) AC | sc-136148 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYP46 Antibody (1F11) HRP | sc-136148 HRP | 200 µg/ml | $316.00 | |||
CYP46 Antibody (1F11) FITC | sc-136148 FITC | 200 µg/ml | $330.00 | |||
CYP46 Antibody (1F11) PE | sc-136148 PE | 200 µg/ml | $343.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 488 | sc-136148 AF488 | 200 µg/ml | $357.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 546 | sc-136148 AF546 | 200 µg/ml | $357.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 594 | sc-136148 AF594 | 200 µg/ml | $357.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 647 | sc-136148 AF647 | 200 µg/ml | $357.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 680 | sc-136148 AF680 | 200 µg/ml | $357.00 | |||
CYP46 Antibody (1F11) Alexa Fluor® 790 | sc-136148 AF790 | 200 µg/ml | $357.00 |