CYP2J6 CRISPR/Cas9 KO Plasmid (m): sc-419921
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP2J6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CYP2J6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP2J6 CRISPR/Cas9 KO Plasmid (m) | sc-419921 | 20 µg | $397.00 |
Overview
Cyp2j6 encodes the cytochrome P450 enzyme CYP2J6, an endoplasmic reticulum–associated monooxygenase that catalyzes oxidative metabolism of endogenous lipids and xenobiotics. CYP2J family enzymes contribute to arachidonic acid epoxygenase activity, generating epoxyeicosatrienoic acids that modulate inflammatory signaling, vascular tone, and cellular stress responses. Through roles in redox balance and lipid mediator homeostasis, Cyp2j6 can influence pathways linked to hepatic and extrahepatic metabolism, immune regulation, and susceptibility to toxicant-induced tissue injury. Altered P450-dependent lipid signaling is frequently leveraged in mouse models to interrogate mechanisms relevant to cardiometabolic dysfunction and inflammatory disease biology.
CYP2J6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cyp2j6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cyp2j6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cyp2j6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CYP2J6 protein expression.
This CRISPR knockout system enables efficient generation of Cyp2j6-deficient cell models for investigation of CYP2J6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cyp2j6 exon(s) critical for CYP2J6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cyp2j6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CYP2J6 CRISPR/Cas9 KO Plasmid (m) and CYP2J6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cyp2j6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CYP2J6 HDR Plasmid (m) and CYP2J6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cyp2j6 homology arms to support homology-directed repair at defined Cyp2j6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.