CYP2F1 Double Nickase Plasmid (h): sc-404064-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP2F1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYP2F1 Double Nickase Plasmid (h) and CYP2F1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYP2F1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYP2F1 Antibody (E-10): sc-377499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP2F1 Double Nickase Plasmid (h) | sc-404064-NIC | 20 µg | $410.00 | |||
CYP2F1 Double Nickase Plasmid (h2) | sc-404064-NIC-2 | 20 µg | $410.00 |
CYP2F1 encodes a cytochrome P450 monooxygenase that catalyzes oxidative metabolism of endogenous and xenobiotic substrates, contributing to cellular detoxification and bioactivation reactions. As part of the P450 redox system, CYP2F1 couples electron transfer from NADPH via cytochrome P450 reductase to support oxygenation chemistry that can influence oxidative stress, electrophile formation, and downstream signaling responses. CYP2F1 activity is relevant to airway and pulmonary biology where metabolic activation of certain inhaled compounds can affect epithelial injury responses and inflammatory processes. Altered regulation of CYP2F1 has been investigated in the context of inter-individual variability in xenobiotic metabolism and susceptibility to chemically induced tissue damage.
CYP2F1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYP2F1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYP2F1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYP2F1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYP2F1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.