CYP27A1 Antibody (G-2): sc-390974
- CYP27A1 Antibody (G-2) is a mouse monoclonal IgG2a κ CYP27A1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 335-451 mapping within an internal region of CYP27A1 of human origin
- CYP27A1 Antibody (G-2) is recommended for detection of CYP27A1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CYP27A1 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CYP27A1 Antibody (G-2) for WB applications. This reagent is now offered in a bundle with CYP27A1 Antibody (G-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
CYP27A1 Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYP27A1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP27A1 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP27A1 plays a crucial role in cholesterol metabolism and is involved in the synthesis of bile acids, which are essential for the digestion and absorption of dietary fats. CYP27A1 catalyzes the conversion of cholesterol to bile acids, thereby regulating cholesterol levels in the body and influencing lipid metabolism. Dysregulation of CYP27A1 can lead to metabolic disorders, highlighting CYP27A1′s importance in maintaining homeostasis. CYP27A1 is localized to the mitochondria in the liver and kidney, where CYP27A1 performs enzymatic functions, making cellular location vital for activity and overall metabolic processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP27A1 Antibody (G-2) References:
- Genetic variants of CYP19 (aromatase) and breast cancer risk. | Kristensen, VN., et al. 2000. Oncogene. 19: 1329-33. PMID: 10713674
- Aromatase and gynecomastia. | Braunstein, GD. 1999. Endocr Relat Cancer. 6: 315-24. PMID: 10731125
- Metabolism of vitamin D(3) by human CYP27A1. | Sawada, N., et al. 2000. Biochem Biophys Res Commun. 273: 977-84. PMID: 10891358
- Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers. | Repa, JJ., et al. 2000. Science. 289: 1524-9. PMID: 10968783
- CYP27A1 Loss Dysregulates Cholesterol Homeostasis in Prostate Cancer. | Alfaqih, MA., et al. 2017. Cancer Res. 77: 1662-1673. PMID: 28130224
- CYP27A1-dependent anti-melanoma activity of limonoid natural products targets mitochondrial metabolism. | Cho, H., et al. 2021. Cell Chem Biol. 28: 1407-1419.e6. PMID: 33794192
- Bile acid homeostasis in female mice deficient in Cyp7a1 and Cyp27a1. | Rizzolo, D., et al. 2021. Acta Pharm Sin B. 11: 3847-3856. PMID: 35024311
- CYP27A1 deficiency promoted osteoclast differentiation. | Fang, Z., et al. 2023. PeerJ. 11: e15041. PMID: 36890868
- 27-Hydroxylation of oncosterone by CYP27A1 switches its activity from pro-tumor to anti-tumor. | Ayadi, S., et al. 2023. J Lipid Res. 64: 100479. PMID: 37981011
- P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. | Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
- P450BM-3; a tale of two domains--or is it three? | Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
- Endocrine disorders associated with inappropriately high aromatase expression. | Bulun, SE., et al. 1997. J Steroid Biochem Mol Biol. 61: 133-9. PMID: 9365182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP27A1 Antibody (G-2) | sc-390974 | 200 µg/ml | $322.00 | |||
CYP27A1 Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-523758 | 200 µg Ab, 40 µg BP | $361.00 | |||
CYP27A1 Antibody (G-2) AC | sc-390974 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CYP27A1 Antibody (G-2) HRP | sc-390974 HRP | 200 µg/ml | $322.00 | |||
CYP27A1 Antibody (G-2) FITC | sc-390974 FITC | 200 µg/ml | $336.00 | |||
CYP27A1 Antibody (G-2) PE | sc-390974 PE | 200 µg/ml | $349.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 488 | sc-390974 AF488 | 200 µg/ml | $364.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 546 | sc-390974 AF546 | 200 µg/ml | $364.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 594 | sc-390974 AF594 | 200 µg/ml | $364.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 647 | sc-390974 AF647 | 200 µg/ml | $364.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 680 | sc-390974 AF680 | 200 µg/ml | $364.00 | |||
CYP27A1 Antibody (G-2) Alexa Fluor® 790 | sc-390974 AF790 | 200 µg/ml | $364.00 |