CYP27A1 Antibody (D-12): sc-393222
- CYP27A1 Antibody (D-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 335-451 mapping within an internal region of CYP27A1 of human origin
- recommended for detection of CYP27A1 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CYP27A1 Antibody (D-12) for WB applications. These reagents are now offered in bundles with CYP27A1 Antibody (D-12) (see ordering information below).
CYP27A1 Antibody (D-12) is a mouse monoclonal IgG1 antibody that detects CYP27A1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP27A1 plays a crucial role in the metabolism of vitamin D3, specifically catalyzing the 25-hydroxylation of vitamin D3, which is essential for the activation of vitamin D and its subsequent biological functions, including calcium homeostasis and bone health. The importance of CYP27A1 extends beyond vitamin D metabolism, as CYP27A1 is also involved in cholesterol metabolism and bile acid synthesis, making CYP27A1 a key player in lipid homeostasis. This enzyme is predominantly localized in the mitochondria of the liver and kidney, where CYP27A1 activity is tightly regulated to ensure proper metabolic function. Dysregulation of CYP27A1 has been linked to various health issues, including vitamin D deficiency and disorders related to cholesterol metabolism, highlighting the significance of CYP27A1 in maintaining overall health. Anti-CYP27A1 antibody (D-12) is an invaluable tool for researchers studying the intricate roles of this enzyme in human physiology and disease.
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CYP27A1 Antibody (D-12) References:
- Clinical and molecular diagnosis of cerebrotendinous xanthomatosis with a review of the mutations in the CYP27A1 gene. | Gallus, GN., et al. 2006. Neurol Sci. 27: 143-9. PMID: 16816916
- Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol. | Ehrhardt, M., et al. 2016. Biol Chem. 397: 513-8. PMID: 26891232
- Intestinal bacterium-derived cyp27a1 prevents colon cancer cell apoptosis. | Ji, YC., et al. 2016. Am J Transl Res. 8: 4434-4439. PMID: 27830027
- CYP27A1 acts on the pre-vitamin D3 photoproduct, lumisterol, producing biologically active hydroxy-metabolites. | Tuckey, RC., et al. 2018. J Steroid Biochem Mol Biol. 181: 1-10. PMID: 29452159
- CYP27A1 inhibits bladder cancer cells proliferation by regulating cholesterol homeostasis. | Liang, Z., et al. 2019. Cell Cycle. 18: 34-45. PMID: 30563407
- Host CYP27A1 expression is essential for ovarian cancer progression. | He, S., et al. 2019. Endocr Relat Cancer. 26: 659-675. PMID: 31048561
- CYP27A1 inhibits proliferation and migration of clear cell renal cell carcinoma via activation of LXRs/ABCA1. | Liang, Z., et al. 2022. Exp Cell Res. 419: 113279. PMID: 35810773
- The tumor-repressing effect of CYP27A1 on renal cell carcinoma by 27-HC arising from cholesterol metabolism. | Zhang, X., et al. 2022. FASEB J. 36: e22499. PMID: 35969149
- TUBB2B facilitates progression of hepatocellular carcinoma by regulating cholesterol metabolism through targeting HNF4A/CYP27A1. | Wang, X., et al. 2023. Cell Death Dis. 14: 179. PMID: 36872411
- Transcriptomic analysis identifies CYP27A1 as a diagnostic marker for the prognosis and immunity in lung adenocarcinoma. | Yin, Y., et al. 2023. BMC Immunol. 24: 37. PMID: 37817081
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP27A1 Antibody (D-12) | sc-393222 | 200 µg/ml | $322.00 | |||
CYP27A1 Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-538170 | 200 µg Ab; 10 µg BP | $361.00 | |||
CYP27A1 Antibody (D-12): m-IgGκ BP-HRP Bundle | sc-535730 | 200 µg Ab; 40 µg BP | $361.00 | |||
CYP27A1 Antibody (D-12): m-IgG1 BP-HRP Bundle | sc-545558 | 200 µg Ab; 20 µg BP | $361.00 |