CYP24 Double Nickase Plasmid (h): sc-401233-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP24 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYP24 Double Nickase Plasmid (h) and CYP24 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYP24A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYP24 Antibody (E-7): sc-365700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP24 Double Nickase Plasmid (h) | sc-401233-NIC | 20 µg | $410.00 | |||
CYP24 Double Nickase Plasmid (h2) | sc-401233-NIC-2 | 20 µg | $410.00 |
CYP24A1 encodes the mitochondrial cytochrome P450 enzyme CYP24A1 (CYP24), a key regulator of vitamin D metabolism that catalyzes 24-hydroxylation and subsequent inactivation of 1,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3. By controlling calcitriol turnover, CYP24A1 modulates VDR-dependent transcriptional programs that influence calcium and phosphate homeostasis, epithelial differentiation, and immune signaling. Altered CYP24A1 activity has been linked to dysregulated vitamin D signaling and disturbances in mineral metabolism, and its expression is frequently evaluated in contexts where VDR pathway balance is perturbed, including cancer biology and inflammatory states. As a mitochondrial P450, CYP24A1 also intersects with cellular redox processes and metabolic adaptation through its dependence on electron transfer partners.
CYP24 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYP24A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYP24A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYP24A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYP24A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.