CYP24 Antibody (E-7): sc-365700
- CYP24 Antibody (E-7) is a mouse monoclonal IgG1 κ CYP24 antibody, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 351-437 mapping near the C-terminus of CYP24 of human origin
- CYP24 Antibody (E-7) is recommended for detection of CYP24 of human origin by WB, IP, IF and ELISA
- Anti-CYP24 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CYP24 Antibody (E-7) for WB applications. These reagents are now offered in bundles with CYP24 Antibody (E-7) (see ordering information below).
CYP24 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP24 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP24 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP24 protein, also known as vitamin D 24-hydroxylase, plays a crucial role in vitamin D metabolism by catalyzing its degradation, which is essential for maintaining calcium homeostasis and bone health. Dysregulation of CYP24 can lead to altered vitamin D levels, potentially resulting in conditions such as osteoporosis or rickets. Additionally, CYP24 is involved in the inactivation of 1,25-dihydroxyvitamin D3, the active form of vitamin D, thereby regulating its biological effects in various tissues. Expression of CYP24 is tightly controlled and can be influenced by factors such as calcium levels and parathyroid hormone, highlighting its importance in the endocrine regulation of calcium and phosphate metabolism. Understanding CYP24 function is vital for elucidating its role in vitamin D metabolism and implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP24 Antibody (E-7) References:
- Aromatase and gynecomastia. | Braunstein, GD. 1999. Endocr Relat Cancer. 6: 315-24. PMID: 10731125
- Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene. | Albertson, DG., et al. 2000. Nat Genet. 25: 144-6. PMID: 10835626
- Metabolism of vitamin D(3) by human CYP27A1. | Sawada, N., et al. 2000. Biochem Biophys Res Commun. 273: 977-84. PMID: 10891358
- Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers. | Repa, JJ., et al. 2000. Science. 289: 1524-9. PMID: 10968783
- Enzymatic studies on the key enzymes of vitamin D metabolism; 1 alpha-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24). | Inouye, K. and Sakaki, T. 2001. Biotechnol Annu Rev. 7: 179-94. PMID: 11686044
- Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b. | Komagata, S., et al. 2009. Mol Pharmacol. 76: 702-9. PMID: 19570947
- Regulation of CYP24 splicing by 1,25-dihydroxyvitamin D₃ in human colon cancer cells. | Peng, X., et al. 2012. J Endocrinol. 212: 207-15. PMID: 22068926
- Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats. | Wang, L., et al. 2016. Nephrology (Carlton). 21: 1063-1068. PMID: 26729468
- CYP24 inhibition as a therapeutic target in FGF23-mediated renal phosphate wasting disorders. | Bai, X., et al. 2016. J Clin Invest. 126: 667-80. PMID: 26784541
- P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. | Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
- P450BM-3; a tale of two domains--or is it three? | Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
- Endocrine disorders associated with inappropriately high aromatase expression. | Bulun, SE., et al. 1997. J Steroid Biochem Mol Biol. 61: 133-9. PMID: 9365182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP24 Antibody (E-7) | sc-365700 | 200 µg/ml | $316.00 | |||
CYP24 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-527393 | 200 µg Ab; 10 µg BP | $354.00 | |||
CYP24 Antibody (E-7): m-IgG1 BP-HRP Bundle | sc-532766 | 200 µg Ab; 20 µg BP | $354.00 | |||
CYP24 Antibody (E-7) AC | sc-365700 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYP24 Antibody (E-7) HRP | sc-365700 HRP | 200 µg/ml | $316.00 | |||
CYP24 Antibody (E-7) FITC | sc-365700 FITC | 200 µg/ml | $330.00 | |||
CYP24 Antibody (E-7) PE | sc-365700 PE | 200 µg/ml | $343.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 488 | sc-365700 AF488 | 200 µg/ml | $357.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 546 | sc-365700 AF546 | 200 µg/ml | $357.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 594 | sc-365700 AF594 | 200 µg/ml | $357.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 647 | sc-365700 AF647 | 200 µg/ml | $357.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 680 | sc-365700 AF680 | 200 µg/ml | $357.00 | |||
CYP24 Antibody (E-7) Alexa Fluor® 790 | sc-365700 AF790 | 200 µg/ml | $357.00 |