CYP21A2 Antibody (B-7): sc-518265
- CYP21A2 Antibody (B-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 130-156 of CYP21A2 of human origin
- recommended for detection of CYP21A2 of human origin, and Cyp21a1 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-CYP21A2 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP21A2 (B-7): sc-518265.
- m-IgG1 BP-HRP is the preferred secondary detection reagent for CYP21A2 Antibody (B-7) for WB applications. This reagent is now offered in a bundle with CYP21A2 Antibody (B-7) (see ordering information below).
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SEE ALSO...
CYP21A2 (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP21A2 of human origin, and Cyp21a1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CYP21A2 antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, and targets the cytochrome P450 family 21 subfamily A member 2 (CYP21A2) protein. CYP21A2 localizes to the endoplasmic reticulum, where CYP21A2 hydroxylates steroids at the 21 position, a critical step in the biosynthesis of steroid hormones such as cortisol and aldosterone. The endoplasmic reticulum′s role as the site of steroid hormone synthesis underscores the importance of CYP21A2′s localization, as CYP21A2 facilitates the proper processing and modification of steroid precursors. Mutations in the CYP21A2 gene are the primary cause of congenital adrenal hyperplasia, an autosomal recessive disorder. Gene conversion events involving the functional CYP21A2 gene and a related pseudogene located nearby account for the majority of cases of steroid 21-hydroxylase deficiency.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP21A2 Antibody (B-7) References:
- Substitutions in the CYP21A2 promoter explain the simple-virilizing form of 21-hydroxylase deficiency in patients harbouring a P30L mutation. | Araujo, RS., et al. 2005. Clin Endocrinol (Oxf). 62: 132-6. PMID: 15670187
- Congenital adrenal hyperplasia: the molecular basis of 21-hydroxylase deficiency in H-2(aw18) mice. | Riepe, FG., et al. 2005. Endocrinology. 146: 2563-74. PMID: 15731361
- The residue E351 is essential for the activity of human 21-hydroxylase: evidence from a naturally occurring novel point mutation compared with artificial mutants generated by single amino acid substitutions. | Krone, N., et al. 2005. J Mol Med (Berl). 83: 561-8. PMID: 15830218
- Validation and clinical application of a locus-specific polymerase chain reaction- and minisequencing-based assay for congenital adrenal hyperplasia (21-hydroxylase deficiency). | Keen-Kim, D., et al. 2005. J Mol Diagn. 7: 236-46. PMID: 15858147
- 21-Hydroxylase and 11beta-hydroxylase mutations in Romanian patients with classic congenital adrenal hyperplasia. | Grigorescu Sido, A., et al. 2005. J Clin Endocrinol Metab. 90: 5769-73. PMID: 16046588
- Difference in transcriptional activity of two homologous CYP21A genes. | Chang, SF. and Chung, BC. 1995. Mol Endocrinol. 9: 1330-6. PMID: 8544841
- P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. | Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
- P450BM-3; a tale of two domains--or is it three? | Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP21A2 Antibody (B-7) | sc-518265 | 200 µg/ml | $316.00 | |||
Antibody (B-7): m-IgG1 BP-HRP Bundle | sc-551797 | 200 µg Ab; 20 µg BP | $354.00 | |||
CYP21A2 Antibody (B-7) AC | sc-518265 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYP21A2 Antibody (B-7) HRP | sc-518265 HRP | 200 µg/ml | $316.00 | |||
CYP21A2 Antibody (B-7) FITC | sc-518265 FITC | 200 µg/ml | $330.00 | |||
CYP21A2 Antibody (B-7) PE | sc-518265 PE | 200 µg/ml | $343.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 488 | sc-518265 AF488 | 200 µg/ml | $357.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 546 | sc-518265 AF546 | 200 µg/ml | $357.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 594 | sc-518265 AF594 | 200 µg/ml | $357.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 647 | sc-518265 AF647 | 200 µg/ml | $357.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 680 | sc-518265 AF680 | 200 µg/ml | $357.00 | |||
CYP21A2 Antibody (B-7) Alexa Fluor® 790 | sc-518265 AF790 | 200 µg/ml | $357.00 |