CYP1B1 Antibody (G-4): sc-374228
- CYP1B1 Antibody (G-4) is a mouse monoclonal IgG1 κ CYP1B1 antibody, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 221-325 mapping within an internal region of CYP1B1 of human origin
- CYP1B1 Antibody (G-4) is recommended for detection of CYP1B1 of human origin by WB, IP, IF and ELISA
- Anti-CYP1B1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for CYP1B1 Antibody (G-4) for WB applications. This reagent is now offered in a bundle with CYP1B1 Antibody (G-4) (see ordering information below).
CYP1B1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP1B1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CYP1B1 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytochrome P450 1B1 (CYP1B1) plays a crucial role in the metabolism of estrogens, particularly in the conversion of 17-beta-estradiol into catechol estrogen metabolites, such as 2-hydroxyestradiol and 4-hydroxyestradiol, which have been implicated in the development of breast cancer. CYP1B1 activity is significant because these metabolites can form DNA adducts, leading to mutations and potentially initiating carcinogenesis in mammary tissues. CYP1B1 is the predominant member of the cytochrome P450 family expressed in normal breast tissue and is upregulated in breast cancer, highlighting CYP1B1′s importance in both normal physiology and disease states. Genetic variations in the CYP1B1 gene, particularly at codon 432, can alter CYP1B1 enzymatic function and may contribute to individual susceptibility to prostate cancer, underscoring CYP1B1′s relevance in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP1B1 Antibody (G-4) References:
- Human CYP1B1 Leu432Val gene polymorphism: ethnic distribution in African-Americans, Caucasians and Chinese; oestradiol hydroxylase activity; and distribution in prostate cancer cases and controls. | Tang, YM., et al. 2000. Pharmacogenetics. 10: 761-6. PMID: 11221602
- Association of CYP1B1 polymorphisms and breast cancer risk. | De Vivo, I., et al. 2002. Cancer Epidemiol Biomarkers Prev. 11: 489-92. PMID: 12010864
- Cytochrome P450 CYP1B1 and catechol O-methyltransferase (COMT) genetic polymorphisms and breast cancer susceptibility in a Turkish population. | Kocabaş, NA., et al. 2002. Arch Toxicol. 76: 643-9. PMID: 12415427
- Interaction between genetic polymorphism of cytochrome P450-1B1 and environmental pollutants in breast cancer risk. | Saintot, M., et al. 2004. Eur J Cancer Prev. 13: 83-6. PMID: 15075793
- Association of cytochrome P450 1B1 (CYP1B1) polymorphism with steroid receptor status in breast cancer. | Bailey, LR., et al. 1998. Cancer Res. 58: 5038-41. PMID: 9823305
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP1B1 Antibody (G-4) | sc-374228 | 200 µg/ml | $316.00 | |||
CYP1B1 Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-531711 | 200 µg Ab; 20 µg BP | $354.00 | |||
CYP1B1 Antibody (G-4) AC | sc-374228 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYP1B1 Antibody (G-4) HRP | sc-374228 HRP | 200 µg/ml | $316.00 | |||
CYP1B1 Antibody (G-4) FITC | sc-374228 FITC | 200 µg/ml | $330.00 | |||
CYP1B1 Antibody (G-4) PE | sc-374228 PE | 200 µg/ml | $343.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 488 | sc-374228 AF488 | 200 µg/ml | $357.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 546 | sc-374228 AF546 | 200 µg/ml | $357.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 594 | sc-374228 AF594 | 200 µg/ml | $357.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 647 | sc-374228 AF647 | 200 µg/ml | $357.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 680 | sc-374228 AF680 | 200 µg/ml | $357.00 | |||
CYP1B1 Antibody (G-4) Alexa Fluor® 790 | sc-374228 AF790 | 200 µg/ml | $357.00 |