CYP1B1 Antibody (G-4) recommended for detection of CYP1B1 of human origin by WB, IP, IF and ELISA

CYP1B1 Antibody (G-4): sc-374228

CYP1B1 Antibody (G-4) is rated 3.6 out of 5 by 7.
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Datasheets

    • Anti-CYP1B1 Antibody (G-4) is a mouse monoclonal IgG1 (kappa light chain) CYP1B1 antibody provided at 200 µg/ml
    • raised against amino acids 221-325 mapping within an internal region of CYP1B1 of human origin
    • CYP1B1 Antibody (G-4) is recommended for detection of CYP1B1 of human origin by WB, IP, IF and ELISA
    • Anti-CYP1B1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP1B1 (G-4): sc-374228.
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hi, i purchase 4 vials of thus and i could not detect band in mcf-7 and t47d ,mda-231 .mda-468 please could yoy help me in this issue as i did not want to change it to other compny please advise me best regards, hend

Asked by: deem
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-05-20

Can CYP1B1 (G-4): sc-374228 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CYP1B1 (G-4): sc-374228 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works well for WB! Worked well when using for WB at 1:200 dilution. Provided nice clear bands in both human and mouse tissue lysates.
Date published: 2018-03-22
Rated 1 out of 5 by from Doesn't work for immunofluorescence on mouse cells Used on mouse ovarian epithelial cells at 1/100 overnight at 4C then 1/500 goat α-mouse secondary for 1H at room temp. No signal detected.
Date published: 2017-03-24
Rated 1 out of 5 by from Ne fonctionne pas chez la souris Ne fonctionne ni en WB sur des extraits de testicules de souris ni en immunohistochimie sur des testicules de souris
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent choice for CYP1B1! Great antibody for CYP1B1 detection: strong and specific, highly reliable.
Date published: 2017-01-20
Rated 4 out of 5 by from Working well so far Working well so far.
Date published: 2015-06-15
Rated 5 out of 5 by from Worked with human transfected lysate by WB Worked with human transfected lysate by WB -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Western blot publishable data in human liver Western blot publishable data in human liver tissue. -SCBT Publication Review
Date published: 2013-04-06
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