Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CYP11B1 Double Nickase Plasmid (h): sc-403040-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP11B1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYP11B1 Double Nickase Plasmid (h) and CYP11B1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYP11B1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYP11B1 Antibody (H-11): sc-374096
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP11B1 Double Nickase Plasmid (h) | sc-403040-NIC | 20 µg | $410.00 | |||
CYP11B1 Double Nickase Plasmid (h2) | sc-403040-NIC-2 | 20 µg | $410.00 |
CYP11B1 encodes a mitochondrial cytochrome P450 enzyme that catalyzes 11β-hydroxylation steps essential for cortisol and corticosterone biosynthesis in the adrenal cortex. As part of the steroidogenic pathway, CYP11B1 functions downstream of cholesterol mobilization and mitochondrial electron transfer systems, integrating ACTH-regulated control of glucocorticoid output. Altered CYP11B1 activity perturbs glucocorticoid homeostasis and adrenal steroid flux, which is relevant to disorders characterized by abnormal cortisol production and compensatory changes in mineralocorticoid and androgen pathways. CYP11B1 is therefore widely studied in adrenal cell models to interrogate steroidogenesis, mitochondrial P450 catalysis, and endocrine stress-response signaling.
CYP11B1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYP11B1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYP11B1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYP11B1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYP11B1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.