CyP Antibody (E-8): sc-390193
- CyP Antibody (E-8) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-208 representing full length CyPB of human origin
- Anti-CyP Antibody (E-8) is recommended for detection of CyPB and CyPC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CyP Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for CyP Antibody (E-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with CyP Antibody (E-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
CyP Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects CyP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CyP (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Cyclophilins, including CyPA and CyPB, play critical roles in cellular processes, with CyPA being particularly important for T cell differentiation and proliferation, which are essential for adaptive immunity. CyPA is primarily located in the cytosol, where CyPA functions as a peptidyl-prolyl cis-trans isomerase, facilitating proper protein folding by accelerating the isomerization of proline-containing peptides. This function is vital for maintaining protein homeostasis and ensuring proteins achieve correct three-dimensional structures, which is crucial for biological activity. Additionally, CyPA interacts with the capsid domain of HIV-1 Gag polyprotein, highlighting CyPA′s significance in viral replication and pathogenesis. Anti-CyP antibody (E-8) detection capabilities make this reagent an invaluable tool for researchers studying immune responses and viral infections.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CyP Antibody (E-8) References:
- Maturation-induced conformational changes of HIV-1 capsid protein and identification of two high affinity sites for cyclophilins in the C-terminal domain. | Endrich, MM., et al. 1999. J Biol Chem. 274: 5326-32. PMID: 10026140
- Two cyclophilin A homologs with shared and distinct functions important for growth and virulence of Cryptococcus neoformans. | Wang, P., et al. 2001. EMBO Rep. 2: 511-8. PMID: 11415984
- Effects of altered cyclophilin A expression on growth and differentiation of human and mouse neuronal cells. | Nahreini, P., et al. 2001. Cell Mol Neurobiol. 21: 65-79. PMID: 11440199
- s-cyclophilin is retained intracellularly via a unique COOH-terminal sequence and colocalizes with the calcium storage protein calreticulin. | Arber, S., et al. 1992. J Cell Biol. 116: 113-25. PMID: 1530944
- An endoplasmic reticulum-specific cyclophilin. | Hasel, KW., et al. 1991. Mol Cell Biol. 11: 3484-91. PMID: 1710767
- CYP 2C19 and UDP-glucuronosyltransferases not only for drugs but also for endobiotics. | Siest, G., et al. 2014. Drug Metabol Drug Interact. 29: 207-9. PMID: 25367614
- CYP-derived eicosanoids in inflammatory diseases. | Tunctan, B. 2020. Prostaglandins Other Lipid Mediat. 148: 106424. PMID: 32006665
- In situ detection of cyclosporin A: evidence for nuclear localization of cyclosporine and cyclophilins. | Le Hir, M., et al. 1995. Lab Invest. 73: 727-33. PMID: 7474947
- X-ray structure of a decameric cyclophilin-cyclosporin crystal complex. | Pflügl, G., et al. 1993. Nature. 361: 91-4. PMID: 8421501
- Evidence that human milk isolated cyclophilin B corresponds to a truncated form. | Mariller, C., et al. 1996. Biochim Biophys Acta. 1293: 31-8. PMID: 8652625
- Involvement of the N-terminal part of cyclophilin B in the interaction with specific Jurkat T-cell binding sites. | Mariller, C., et al. 1996. Biochem J. 317 (Pt 2): 571-6. PMID: 8713087
- Crystal structure of cyclophilin A complexed with a binding site peptide from the HIV-1 capsid protein. | Vajdos, FF., et al. 1997. Protein Sci. 6: 2297-307. PMID: 9385632
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CyP Antibody (E-8) | sc-390193 | 200 µg/ml | $322.00 | |||
CyP Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-523442 | 200 µg Ab, 40 µg BP | $361.00 | |||
CyP Antibody (E-8) AC | sc-390193 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CyP Antibody (E-8) HRP | sc-390193 HRP | 200 µg/ml | $322.00 | |||
CyP Antibody (E-8) FITC | sc-390193 FITC | 200 µg/ml | $336.00 | |||
CyP Antibody (E-8) PE | sc-390193 PE | 200 µg/ml | $349.00 | |||
CyP Antibody (E-8) Alexa Fluor® 488 | sc-390193 AF488 | 200 µg/ml | $364.00 | |||
CyP Antibody (E-8) Alexa Fluor® 546 | sc-390193 AF546 | 200 µg/ml | $364.00 | |||
CyP Antibody (E-8) Alexa Fluor® 594 | sc-390193 AF594 | 200 µg/ml | $364.00 | |||
CyP Antibody (E-8) Alexa Fluor® 647 | sc-390193 AF647 | 200 µg/ml | $364.00 | |||
CyP Antibody (E-8) Alexa Fluor® 680 | sc-390193 AF680 | 200 µg/ml | $364.00 | |||
CyP Antibody (E-8) Alexa Fluor® 790 | sc-390193 AF790 | 200 µg/ml | $364.00 |