Anti-cyclin T2a/b Antibody (2128C1a) recommended for detection of cyclin T2a and cyclin T2b of human origin by WB and IP

Anti-cyclin T2a/b Antibody (2128C1a): sc-81243

Anti-cyclin T2a/b Antibody (2128C1a) is rated 4.3 out of 5 by 4.
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Datasheets

    • Anti-cyclin T2a/b Antibody (2128C1a) is a mouse monoclonal IgG1 cyclin T2a/b antibody, cited in 4 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of cyclin T2a/b of human origin
    • recommended for detection of cyclin T2a and cyclin T2b of human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin T2a/b (2128C1a): sc-81243.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin T2a/b Antibody (2128C1a) for WB applications. These reagents are now offered in bundles with cyclin T2a/b Antibody (2128C1a) (see ordering information below).
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I am using cyclin T2a/b (2128C1a): sc-81243 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from good product Clean double band appearing at ~100 & ~80 kDa in HeLa whole cell lysate.
Date published: 2018-04-26
Rated 4 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), albeit with a higher background
Date published: 2017-09-18
Rated 4 out of 5 by from good antibody it is a good antibody for western blot analysis when I tested it in lung cancer cell lines.
Date published: 2017-08-29
Rated 4 out of 5 by from Band at expected size but faint Band at expected size but faint. WB data in NIH/3T3. -SCBT QC
Date published: 2013-07-08
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