cyclin O (h): 293T Lysate: sc-173001

Cyclin O (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, enhancing its transfection efficiency and protein production capabilities. This lysate is specifically enriched with human cyclin O, a member of the cyclin family of proteins that regulate the cell cycle. Cyclin O is involved in the control of the G2/M transition and has been implicated in the activation of the cyclin-dependent kinase (CDK) pathways that are essential for cell cycle progression. In research, cyclin O (h): 293T Lysate is utilized to study the molecular mechanisms of cell cycle regulation and the specific role of cyclin O in these processes. Researchers employ this lysate in Western blotting to detect and quantify cyclin O expression levels under various experimental conditions, providing insights into its regulation and function during cell cycle transitions. The lysate is also used in co-immunoprecipitation assays to identify protein-protein interactions involving cyclin O, which helps to explain its role in forming active cyclin-CDK complexes. Functional assays using cyclin O (h): 293T Lysate can assess the impact of cyclin O on CDK activity and cell cycle progression, contributing to a deeper understanding of how cyclin O influences cell division and growth. These studies are crucial for unraveling the regulatory networks that ensure proper cell cycle control and prevent aberrant cell proliferation.