cyclin G1 CRISPR/Cas9 KO Plasmid (h): sc-402045
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin G1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cyclin G1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin G1 Antibody (F-5): sc-8016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin G1 CRISPR/Cas9 KO Plasmid (h) | sc-402045 | 20 µg | $397.00 |
Overview
CCNG1 encodes cyclin G1, an atypical cyclin that is rapidly induced by cellular stress and integrates signaling from the p53 network to modulate cell-cycle progression and checkpoint control. Cyclin G1 participates in feedback regulation of p53 activity through phosphatase-associated complexes, influencing phosphorylation-dependent decisions in DNA damage responses. Through these functions it impacts proliferation, genomic stability, and apoptosis-related signaling programs. Dysregulated CCNG1 expression or p53-pathway context dependence has been observed across multiple cancer types, making it a useful node for studying oncogenic stress responses and tumor cell fitness.
cyclin G1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCNG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCNG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCNG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cyclin G1 protein expression.
This CRISPR knockout system enables efficient generation of CCNG1-deficient cell models for investigation of cyclin G1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCNG1 exon(s) critical for cyclin G1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCNG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cyclin G1 CRISPR/Cas9 KO Plasmid (h) and cyclin G1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCNG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cyclin G1 HDR Plasmid (h) and cyclin G1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCNG1 homology arms to support homology-directed repair at defined CCNG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.