cyclin G1 Antibody (F-5): sc-8016

Cyclin G1 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin G1 of human origin through applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. Cyclin G1 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cyclin G1 plays a crucial role in cell cycle regulation, particularly in the transition from G1 phase to S phase, where cyclin G1 is involved in the control of cell proliferation. Cyclin G1 is essential for proper cell cycle progression, helping to ensure cells only replicate DNA when ready, thereby preventing uncontrolled cell division that can lead to cancer. Cyclin G1 is characterized by a typical N-terminal cyclin box and a C-terminal domain homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G1 expression is induced within three hours following growth stimulation and remains elevated without clear dependency on the cell cycle, distinguishing cyclin G1 from other cyclins that exhibit more regulated expression patterns. Cyclin G2, which shares 53% amino acid sequence identity with cyclin G1, peaks in expression during late S phase, further highlighting unique regulatory roles these proteins play in cell cycle dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin G1 Antibody (F-5) References:

1. D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA.  |  Xiong, Y., et al. 1992. Cell. 71: 505-14. PMID: 1358458
2. Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins.  |  Matsushime, H., et al. 1992. Cell. 71: 323-34. PMID: 1423597
3. Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.  |  Fang, F. and Newport, JW. 1991. Cell. 66: 731-42. PMID: 1652371
4. [Cyclin G1 expressing in mouse ovary and relating to follicular development].  |  Liu, ZH., et al. 2006. Sichuan Da Xue Xue Bao Yi Xue Ban. 37: 893-7. PMID: 17236588
5. Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.  |  Koff, A., et al. 1991. Cell. 66: 1217-28. PMID: 1833068
6. Cyclin A is required for the onset of DNA replication in mammalian fibroblasts.  |  Girard, F., et al. 1991. Cell. 67: 1169-79. PMID: 1836977
7. Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.  |  Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
8. Cyclin G1-mediated epithelial-mesenchymal transition via phosphoinositide 3-kinase/Akt signaling facilitates liver cancer progression.  |  Wen, W., et al. 2012. Hepatology. 55: 1787-98. PMID: 22271581
9. Silencing the Expression of Cyclin G1 Enhances the Radiosensitivity of Hepatocellular Carcinoma In Vitro and In Vivo by Inducing Apoptosis.  |  Xu, G., et al. 2021. Radiat Res. 195: 378-384. PMID: 33543294
10. Cyclin G1 induces maladaptive proximal tubule cell dedifferentiation and renal fibrosis through CDK5 activation.  |  Taguchi, K., et al. 2022. J Clin Invest. 132: PMID: 36453545
11. Cyclin G: a new mammalian cyclin with homology to fission yeast Cig1.  |  Tamura, K., et al. 1993. Oncogene. 8: 2113-8. PMID: 8336937
12. Cyclin G1 and cyclin G2 comprise a new family of cyclins with contrasting tissue-specific and cell cycle-regulated expression.  |  Horne, MC., et al. 1996. J Biol Chem. 271: 6050-61. PMID: 8626390

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin G1 Antibody (F-5) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin G1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin G1 Antibody (F-5) Specifications

Alternate Product Name: cyclin G1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-46

Epitope Target: cyclin G1

Epitope Species: human

Product Reactivity/Detection: cyclin G1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for cyclin G1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin