cyclin E2 Antibody (A-9): sc-28351
- cyclin E2 Antibody (A-9) is a mouse monoclonal IgG2a κ cyclin E2 antibody, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 1-140 of cyclin E2 of human origin
- cyclin E2 Antibody (A-9) is recommended for detection of cyclin E2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyclin E2 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cyclin E2 Antibody (A-9) for WB applications. These reagents are now offered in bundles with cyclin E2 Antibody (A-9) (see ordering information below).
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cyclin E2 Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects cyclin E2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-cyclin E2 antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyclin E2 plays a crucial role in cell cycle regulation, particularly during the G1/S phase transition, where cyclin E2 interacts with cyclin-dependent kinases Cdk2 and Cdk3, as well as cyclin-dependent kinase inhibitors p27 and p21. This interaction is vital for proper cell cycle progression, as cyclin E2 helps regulate Cdk2 activity, which is essential for transitioning from G1 phase to S phase, where DNA replication occurs. Notably, cyclin E2 expression remains low in non-transformed cells but shows significant elevation in tumor-derived cells, highlighting cyclin E2′s potential as a cancer progression biomarker. cyclin E2′s ability to modulate cell cycle dynamics makes cyclin E2 an important target for research into cell proliferation and cancer biology, providing insights into therapeutic strategies for cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin E2 Antibody (A-9) References:
- Cyclin E2, the cycle continues. | Payton, M. and Coats, S. 2002. Int J Biochem Cell Biol. 34: 315-20. PMID: 11854029
- Deregulation of cyclin E2 expression and associated kinase activity in primary breast tumors. | Payton, M., et al. 2002. Oncogene. 21: 8529-34. PMID: 12466974
- Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner. | Lees, E., et al. 1992. Genes Dev. 6: 1874-85. PMID: 1398067
- Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast. | Lew, DJ., et al. 1991. Cell. 66: 1197-206. PMID: 1833066
- Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. | Koff, A., et al. 1991. Cell. 66: 1217-28. PMID: 1833068
- Cyclin D1 and cyclin E2 are differentially expressed in gastric cancer. | Kumari, S., et al. 2016. Med Oncol. 33: 40. PMID: 27034264
- Cyclin E1 and cyclin E2 in ER+ breast cancer: prospects as biomarkers and therapeutic targets. | Milioli, HH., et al. 2020. Endocr Relat Cancer. 27: R93-R112. PMID: 32061162
- Cyclin E2 Promotes Whole Genome Doubling in Breast Cancer. | Lee, C., et al. 2020. Cancers (Basel). 12: PMID: 32823571
- SIRT3-dependent delactylation of cyclin E2 prevents hepatocellular carcinoma growth. | Jin, J., et al. 2023. EMBO Rep. 24: e56052. PMID: 36896611
- Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle. | Lauper, N., et al. 1998. Oncogene. 17: 2637-43. PMID: 9840927
- Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. | Zariwala, M., et al. 1998. Oncogene. 17: 2787-98. PMID: 9840943
- Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers. | Gudas, JM., et al. 1999. Mol Cell Biol. 19: 612-22. PMID: 9858585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin E2 Antibody (A-9) | sc-28351 | 200 µg/ml | $322.00 | |||
cyclin E2 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-525524 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin E2 Antibody (A-9): m-IgG2a BP-HRP Bundle | sc-546303 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin E2 Antibody (A-9) AC | sc-28351 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin E2 Antibody (A-9) HRP | sc-28351 HRP | 200 µg/ml | $322.00 | |||
cyclin E2 Antibody (A-9) FITC | sc-28351 FITC | 200 µg/ml | $336.00 | |||
cyclin E2 Antibody (A-9) PE | sc-28351 PE | 200 µg/ml | $349.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 488 | sc-28351 AF488 | 200 µg/ml | $364.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 546 | sc-28351 AF546 | 200 µg/ml | $364.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 594 | sc-28351 AF594 | 200 µg/ml | $364.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 647 | sc-28351 AF647 | 200 µg/ml | $364.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 680 | sc-28351 AF680 | 200 µg/ml | $364.00 | |||
cyclin E2 Antibody (A-9) Alexa Fluor® 790 | sc-28351 AF790 | 200 µg/ml | $364.00 |