cyclin D2 Antibody (DCS-3): sc-56305

Cyclin D2 Antibody (DCS-3) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect cyclin D2, an essential regulator of the cell cycle. Cyclin D2, also referred to as Ccnd2, cD2, Vin1, and Vin-1, plays a pivotal role in controlling the transition from the G1 phase to the S phase by forming complexes with cyclin-dependent kinases (Cdk4 and Cdk6) within the nucleus, where it phosphorylates the retinoblastoma (Rb) protein, leading to the activation of E2F transcription factors that are crucial for DNA synthesis and cell proliferation. The precise nuclear localization of cyclin D2 is vital for its function in driving cell cycle progression, making cyclin D2 a key player in normal cellular growth and an important factor in the development of various cancers when dysregulated. Anti-cyclin D2 antibody (DCS-3) is raised against the full-length human cyclin D2 protein and exhibits robust reactivity across mouse, rat, and human species in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, cyclin D2 (DCS-3) antibody offers versatility for diverse research applications. By enabling accurate detection and study of cyclin D2′s localization and function within the cell, cyclin D2 monoclonal antibody (DCS-3) supports advancements in understanding cell cycle regulation and the molecular mechanisms underlying oncogenesis, making cyclin D2 (DCS-3) antibody an invaluable tool for cancer research and therapeutic development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin D2 Antibody (DCS-3) References:

1. Cloning of a D-type cyclin from murine erythroleukemia cells.  |  Kiyokawa, H., et al. 1992. Proc Natl Acad Sci U S A. 89: 2444-7. PMID: 1372445
2. Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene.  |  Motokura, T., et al. 1992. J Biol Chem. 267: 20412-5. PMID: 1383201
3. Genomic organization, chromosomal localization, and independent expression of human cyclin D genes.  |  Inaba, T., et al. 1992. Genomics. 13: 565-74. PMID: 1386335
4. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins.  |  Xiong, Y., et al. 1992. Genomics. 13: 575-84. PMID: 1386336
5. Growth-regulated expression of D-type cyclin genes in human diploid fibroblasts.  |  Won, KA., et al. 1992. Proc Natl Acad Sci U S A. 89: 9910-4. PMID: 1409718
6. Human D-type cyclin.  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
7. Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation.  |  Draetta, G. 1990. Trends Biochem Sci. 15: 378-83. PMID: 2147521
8. Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.  |  Ewen, ME., et al. 1993. Cell. 73: 487-97. PMID: 8343202
9. Physical interaction of the retinoblastoma protein with human D cyclins.  |  Dowdy, SF., et al. 1993. Cell. 73: 499-511. PMID: 8490963

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin D2 Antibody (DCS-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin D2 Antibody (DCS-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin D2 Antibody (DCS-3) Specifications

Alternate Product Name: cyclin D2

Product Clone Name: DCS-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: cyclin D2

Epitope Species: human

Product Reactivity/Detection: cyclin D2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for cyclin D2 Antibody (DCS-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred cyclin D1 antibody is cyclin D1 Antibody (DCS-6): sc-20044.