CYB561D2 Antibody (C-2): sc-373870
- CYB561D2 Antibody (C-2) is a mouse monoclonal IgG1 κ CYB561D2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 201-231 within a C-terminal cytoplasmic domain of CYB561D2 of human origin
- CYB561D2 Antibody (C-2) is recommended for detection of CYB561D2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CYB561D2 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CYB561D2 Antibody (C-2) for WB applications. These reagents are now offered in bundles with CYB561D2 Antibody (C-2) (see ordering information below).
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CYB561D2 Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYB561D2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CYB561D2 antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYB561D2, also known as putative tumor suppressor protein 101F6, is a highly hydrophobic protein consisting of 222 amino acids and features six membrane-spanning domains. CYB561D2 is primarily localized to the endosomes and endoplasmic reticulum within the perinuclear region, where CYB561D2 plays a crucial role in reducing ferric ions through two heme-binding sites. This reduction process is vital for maintaining iron homeostasis within cells, which is essential for various cellular functions, including oxygen transport and electron transfer in metabolic pathways. CYB561D2 is ubiquitously expressed, with highest levels found in the lung, liver, and kidney, and CYB561D2 expression is significantly diminished in most lung cancers, suggesting a potential tumor-suppressive function. The gene encoding CYB561D2 is located on human chromosome 3p21.3, a region frequently associated with genetic alterations and early allele loss in numerous human cancers. Furthermore, exogenous expression of CYB561D2 has been shown to induce caspase-independent apoptotic and autophagic pathways, leading to synergistic killing of tumor cells, highlighting CYB561D2′s potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYB561D2 Antibody (C-2) References:
- The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The International Lung Cancer Chromosome 3p21.3 Tumor Suppressor Gene Consortium. | Lerman, MI. and Minna, JD. 2000. Cancer Res. 60: 6116-33. PMID: 11085536
- Expression of several genes in the human chromosome 3p21.3 homozygous deletion region by an adenovirus vector results in tumor suppressor activities in vitro and in vivo. | Ji, L., et al. 2002. Cancer Res. 62: 2715-20. PMID: 11980673
- RASSF1A is a target tumor suppressor from 3p21.3 in nasopharyngeal carcinoma. | Chow, LS., et al. 2004. Int J Cancer. 109: 839-47. PMID: 15027117
- Molecular analysis of deletions in human chromosome 3p21 and the role of resident cancer genes in disease. | Angeloni, D. 2007. Brief Funct Genomic Proteomic. 6: 19-39. PMID: 17525073
- Evaluation of the 3p21.3 tumour-suppressor gene cluster. | Hesson, LB., et al. 2007. Oncogene. 26: 7283-301. PMID: 17533367
- Tumor suppressor 101F6 and ascorbate synergistically and selectively inhibit non-small cell lung cancer growth by caspase-independent apoptosis and autophagy. | Ohtani, S., et al. 2007. Cancer Res. 67: 6293-303. PMID: 17616688
- Involvement of 101F6, a homologue of cytochrome b561, in the reduction of ferric ions. | Mizutani, A., et al. 2007. J Biochem. 142: 699-705. PMID: 17938141
- Functional expression and characterization of human 101F6 protein, a homologue of cytochrome b561 and a candidate tumor suppressor gene product. | Recuenco, MC., et al. 2008. Biofactors. 34: 219-30. PMID: 19734123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYB561D2 Antibody (C-2) | sc-373870 | 200 µg/ml | $316.00 | |||
CYB561D2 Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-527421 | 200 µg Ab; 10 µg BP | $354.00 | |||
CYB561D2 Antibody (C-2): m-IgG1 BP-HRP Bundle | sc-532794 | 200 µg Ab; 20 µg BP | $354.00 | |||
CYB561D2 Antibody (C-2) AC | sc-373870 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYB561D2 Antibody (C-2) HRP | sc-373870 HRP | 200 µg/ml | $316.00 | |||
CYB561D2 Antibody (C-2) FITC | sc-373870 FITC | 200 µg/ml | $330.00 | |||
CYB561D2 Antibody (C-2) PE | sc-373870 PE | 200 µg/ml | $343.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 488 | sc-373870 AF488 | 200 µg/ml | $357.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 546 | sc-373870 AF546 | 200 µg/ml | $357.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 594 | sc-373870 AF594 | 200 µg/ml | $357.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 647 | sc-373870 AF647 | 200 µg/ml | $357.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 680 | sc-373870 AF680 | 200 µg/ml | $357.00 | |||
CYB561D2 Antibody (C-2) Alexa Fluor® 790 | sc-373870 AF790 | 200 µg/ml | $357.00 | |||
CYB561D2 (C-2) Neutralizing Peptide | sc-373870 P | 100 µg/0.5 ml | $68.00 |