CXXC5 Double Nickase Plasmid (h): sc-403248-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXXC5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CXXC5 Double Nickase Plasmid (h) and CXXC5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXXC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CXXC5 Antibody (H-6): sc-376348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXXC5 Double Nickase Plasmid (h) | sc-403248-NIC | 20 µg | $410.00 | |||
CXXC5 Double Nickase Plasmid (h2) | sc-403248-NIC-2 | 20 µg | $410.00 |
CXXC5 (CXXC-type zinc finger protein 5) is a nuclear and cytoplasmic regulator that binds CpG-rich DNA and interfaces with chromatin-associated complexes to modulate transcriptional programs. It functions as a feedback inhibitor of canonical Wnt/β-catenin signaling through interaction with Dishevelled, thereby influencing cell fate decisions, proliferation, and differentiation. CXXC5 has also been linked to stress-responsive pathways and regulation of cellular identity programs, making it relevant to studies of developmental biology and tissue homeostasis. Dysregulated CXXC5 expression or signaling has been associated with altered Wnt pathway outputs observed in cancer and other proliferative disorders, supporting its use as a mechanistic node in pathway-focused research.
CXXC5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXXC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXXC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXXC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXXC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.