CXX1 CRISPR/Cas9 KO Plasmid (h): sc-406246
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXX1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CXX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXX1 CRISPR/Cas9 KO Plasmid (h) | sc-406246 | 20 µg | $397.00 |
Overview
FAM127A encodes the human protein CXX1, a poorly characterized factor implicated in nuclear processes and cell state regulation. Available evidence suggests CXX1 may participate in chromatin-associated functions and transcriptional control, linking it to pathways that influence proliferation, stress responses, and differentiation programs. Altered expression of FAM127A has been reported in genomic and transcriptomic datasets from multiple disease contexts, supporting its value as a research target for investigating gene regulation mechanisms and cell fitness phenotypes.
CXX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM127A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM127A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM127A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CXX1 protein expression.
This CRISPR knockout system enables efficient generation of FAM127A-deficient cell models for investigation of CXX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM127A exon(s) critical for CXX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM127A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CXX1 CRISPR/Cas9 KO Plasmid (h) and CXX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM127A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CXX1 HDR Plasmid (h) and CXX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM127A homology arms to support homology-directed repair at defined FAM127A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.