CXCL16 Double Nickase Plasmid (h): sc-405248-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXCL16 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CXCL16 Double Nickase Plasmid (h) and CXCL16 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXCL16. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CXCL16 Antibody (C-5): sc-514363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXCL16 Double Nickase Plasmid (h) | sc-405248-NIC | 20 µg | $410.00 | |||
CXCL16 Double Nickase Plasmid (h2) | sc-405248-NIC-2 | 20 µg | $410.00 |
CXCL16 encodes a transmembrane chemokine that can be proteolytically shed to generate a soluble ligand for CXCR6, coordinating chemotaxis and retention of activated T cells, NK cells, and other leukocyte subsets. Through CXCR6-dependent signaling, CXCL16 contributes to leukocyte trafficking, adhesion, and inflammatory crosstalk within tissues, linking chemokine gradients to immune surveillance and tissue remodeling. The membrane-bound form also functions as a scavenger receptor for oxidized lipids and apoptotic debris, connecting innate immune recognition with inflammatory signaling pathways. Dysregulated CXCL16/CXCR6 axis activity has been associated with chronic inflammatory conditions, atherosclerosis-related processes, and tumor immune microenvironment dynamics, making it a relevant target for mechanistic studies of immunopathology.
CXCL16 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXCL16 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXCL16. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXCL16 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXCL16-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.